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  • 产品名称：CD46Molecule,ComplementRegulatoryProtein(CD46)(AA35-328)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CD46Molecule,ComplementRegulatoryProtein(CD46)(AA35-328)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 33.6 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Complement regulatory protein CD46 is also known as membrane Cofactor Protein (MCP), is a type I membrane protein and is a regulatory part of the complement system. CD46 is expressed by all cells except erythrocytes. MCP acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue, and also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. In T-cells by binding to CD46, A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype. Defects in CD46 are a cause of susceptibility to hemolytic uremic syndrome atypical type 2 (AHUS2).
Molecular Weight 33.6 kDa
UniProt P15529
Research Area Immunology, Innate Immunity, CD Antigens, Surface Receptors of Immune Cells
Pathways Regulation of Actin Filament Polymerization
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for CD46 Molecule, Complement Regulatory Protein (CD46) (AA 35-328) (Active) protein (His tag) (ABIN2180802) Human CD46, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ove...
Background publications Noris, Brioschi, Caprioli, Todeschini, Bresin, Porrati, Gamba, Remuzzi: "Familial haemolytic uraemic syndrome and an MCP mutation." in: Lancet, Vol. 362, Issue 9395, pp. 1542-7, 2003 (PubMed).

Kemper, Chan, Green, Brett, Murphy, Atkinson: "Activation of human CD4+ cells with CD3 and CD46 induces a T-regulatory cell 1 phenotype." in: Nature, Vol. 421, Issue 6921, pp. 388-92, 2003 (PubMed).

Caprioli, Castelletti, Bucchioni, Bettinaglio, Bresin, Pianetti, Gamba, Brioschi, Daina, Remuzzi, Noris: "Complement factor H mutations and gene polymorphisms in haemolytic uraemic syndrome: the C-257T, the A2089G and the G2881T polymorphisms are strongly associated with the disease." in: Human molecular genetics, Vol. 12, Issue 24, pp. 3385-95, 2003 (PubMed).

Astier, Trescol-Biémont, Azocar, Lamouille, Rabourdin-Combe: "Cutting edge: CD46, a new costimulatory molecule for T cells, that induces p120CBL and LAT phosphorylation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 12, pp. 6091-5, 2000 (PubMed).

Lublin, Liszewski, Post, Arce, Le Beau, Rebentisch, Lemons, Seya, Atkinson: "Molecular cloning and chromosomal localization of human membrane cofactor protein (MCP). Evidence for inclusion in the multigene family of complement-regulatory proteins." in: The Journal of experimental medicine, Vol. 168, Issue 1, pp. 181-94, 1988 (PubMed).