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  • 产品名称：Interleukin-6Receptor(IL6R)(AA20-365)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
Interleukin-6Receptor(IL6R)(AA20-365)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 40 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >92 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA.Immobilized Human IL6R /CD126 Protein (Cat# ILR-H4223) at 5μg/mL (100 μL/well),can bind Biotinylated rh IL6 Protein (Cat# IL6-H8218).The EC50 is typically 0.06-0.6 μg/mL. Measured by its ability to enhance the IL6 activity on M1 mouse myeloid leukemia cells.The ED50 for this effect is typically 15-60 ng/mL.

SDS-PAGE: due to glycosylation.
Background Interleukin 6 receptor (IL6R) is also known as CD126 (Cluster of Differentiation 126) , is a potent pleiotropic cytokine that regulates cell growth and differentiation of various tissues, and is known particularly for its role in the immune response and acute phase reactions. IL6R is a protein complex consisting of a IL-6 receptor subunit (IL6R) and interleukin 6 signal transducer Glycoprotein 130. IL6R also denotes the human gene encoding this subunit. Alternatively spliced transcript variants encoding distinct isoforms have been reported. IL6R subunit also shared by many other cytokines. The soluble form of IL6R arises from proteolytic cleavage of membrane-bound IL6Rα, and acts agonistically by making the IL6 ligand accessible to the signal transducer gp130. Dysregulated production of IL6 and IL6R are implicated in the pathogenesis of several inflammatory diseases and malignancies such as multiple myeloma, rheumatoid arthritis, or osteoporosis, and it has been reported that a humanized anti-IL6R monoclonal antibody is a promising agent applicable to the therapeutic approach for IL6 driven diseases. Interleukin-6 receptor has been shown to interact with Interleukin 6 and Ciliary neurotrophic factor.
Molecular Weight 40 kDa
NCBI Accession NP_000556
UniProt P08887
Pathways JAK-STAT Signaling, Autophagy, Growth Factor Binding
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Interleukin-6 Receptor (IL6R) (AA 20-365) (Active) protein (His tag) (ABIN2181394) Human IL-6 R alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was sta...
Background publications Egler, Burlingame, Nuchtern, Russell: "Interleukin-6 and soluble interleukin-6 receptor levels as markers of disease extent and prognosis in neuroblastoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 21, pp. 7028-34, 2008 (PubMed).

Yoshio-Hoshino, Adachi, Aoki, Pereboev, Curiel, Nishimoto: "Establishment of a new interleukin-6 (IL-6) receptor inhibitor applicable to the gene therapy for IL-6-dependent tumor." in: Cancer research, Vol. 67, Issue 3, pp. 871-5, 2007 (PubMed).