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  • 产品名称：CathepsinE(CTSE)(AA20-396)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CathepsinE(CTSE)(AA20-396)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 41.6 kDa. The protein migrates as 40-46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2.

SDS-PAGE: due to glycosylation.
Background Cathepsin E (CTSE) is also known as Cd152,Ly56 and Ctla4,which can be cleaved into the following 2 chains: Cathepsin E form I and Cathepsin E form II. Cathepsin E belongs to the peptidase A1 family. CTSE is similar to cathepsin D, but slightly broader specificity. The proenzyme is localized to the endoplasmic reticulum and Golgi apparatus, while the mature enzyme is localized to the endosome. CTSE is expressed abundantly in the stomach, the Clara cells of the lung and activated B-lymphocytes, and at lower levels in lymph nodes, skin and spleen, but not expressed in resting B-lymphocytes. CTSE may have a role in immune function,activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain.At the same time, CTSE is also probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation.
Molecular Weight 41.6 kDa
UniProt P14091
Research Area Immunology, Adaptive Immunity, Innate Immunity, Proteases
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Cathepsin E (CTSE) (AA 20-396) (Active) protein (His tag) (ABIN2180946) Human Cathepsin E, His Tag on SDS-PAGE under reducing (R) condition. The gel was stai...
Background publications Sealy, Mota, Rayment, Tatnell, Kay, Chain: "Regulation of cathepsin E expression during human B cell differentiation in vitro." in: European journal of immunology, Vol. 26, Issue 8, pp. 1838-43, 1996 (PubMed).

Finley, Kornfeld: "Subcellular localization and targeting of cathepsin E." in: The Journal of biological chemistry, Vol. 269, Issue 49, pp. 31259-66, 1995 (PubMed).

Athauda, Matsuzaki, Kageyama, Takahashi: "Structural evidence for two isozymic forms and the carbohydrate attachment site of human gastric cathepsin E." in: Biochemical and biophysical research communications, Vol. 168, Issue 2, pp. 878-85, 1990 (PubMed).