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  • 产品名称：PhospholipaseA2(YWHAZ)(AA23-148)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
PhospholipaseA2(YWHAZ)(AA23-148)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15 kDa. The protein migrates as 19 kDa under reducing (R) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to hydrolyze 1-Hexadecanoyl-2-(1-pyrene-decanoyl)-sn-glycero-3-phosphoglycerol. The specific activity is >2,500 pmol/min/μg.
Background Phosphatidylcholine 2-acylhydrolase 1B (PLA2G1B) is also known as Phospholipase A2 (PLA2), PLA2A, PPLA2, which belongs to the phospholipase A2 family. PLA2G1B is activated by trypsin cleavage in the duodenum and can also be activated by thrombin or autocatalytically. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides, this releases glycerophospholipids and arachidonic acid that serve as the precursors of signal molecules.
Molecular Weight 15 kDa
UniProt P04054
Research Area Signaling, Metabolism, Enzymes
Pathways Inositol Metabolic Process
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Sodium Acetate, 100 mM NaCl, pH 4.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Phospholipase A2 (YWHAZ) (AA 23-148) (Active) protein (His tag) (ABIN2181652) Human PLA2G1B, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ...
Background publications Xu, Yi, Feng, Chen, Liu: "Structural insight into the activation mechanism of human pancreatic prophospholipase A2." in: The Journal of biological chemistry, Vol. 284, Issue 24, pp. 16659-66, 2009 (PubMed).

Grataroli, Dijkman, Dutilh, van der Ouderaa, De Haas, Figarella: "Studies on prophospholipase A2 and its enzyme from human pancreatic juice. Catalytic properties and sequence of the N-terminal region." in: European journal of biochemistry / FEBS, Vol. 122, Issue 1, pp. 111-7, 1982 (PubMed).