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  • 产品名称：CystatinD(CST5)(AA21-142)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CystatinD(CST5)(AA21-142)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 14.7 kDa. The protein migrates as 15 kDa under reducing (R) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC. The IC50 value is <15 nm.="" <="" td="">
Background Cystatin-5 (CST5) is also known as Cystatin-D, is a secreted thermostable protein, which belongs to the cystatin family. CST5 is a cysteine proteinase inhibitor that possibly plays a protective role against proteinases present in the oral cavity. The order of preference for inhibition is cathepsin S > cathepsin H > cathepsin L > cathepsin B. CST5 / cystatin-D may play a protective role against proteinases present in the oral cavity.
Molecular Weight 14.7 kDa
UniProt P28325
Research Area Proteolysis / Ubiquitin
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Cystatin D (CST5) (AA 21-142) (Active) protein (His tag) (ABIN2180922) Human Cystatin D, His Tag on SDS-PAGE under reducing (R) condition. The gel was stain...
Background publications Ryan, Souda, Halgand, Wong, Loo, Faull, Whitelegge: "Confident assignment of intact mass tags to human salivary cystatins using top-down Fourier-transform ion cyclotron resonance mass spectrometry." in: Journal of the American Society for Mass Spectrometry, Vol. 21, Issue 6, pp. 908-17, 2010 (PubMed).

Balbín, Hall, Grubb, Mason, López-Otín, Abrahamson: "Structural and functional characterization of two allelic variants of human cystatin D sharing a characteristic inhibition spectrum against mammalian cysteine proteinases." in: The Journal of biological chemistry, Vol. 269, Issue 37, pp. 23156-62, 1994 (PubMed).