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  • 产品名称：CarboxypeptidaseM(CPM)(AA18-422)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CarboxypeptidaseM(CPM)(AA18-422)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 47.1 kDa. The protein migrates as 49 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >90 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to release Larginine from BenzoylAlaArg, with detection of the arginine amino group by ophthaldialdehyde. The specific activity is >40,000 pmoles/min/μg.

SDS-PAGE: due to glycosylation.
Background Carboxypeptidase M (CPM) belongs to the peptidase M14 family. CPM specifically removes C-terminal basic residues (Arg or Lys) from peptides and proteins, and the activity is inhibited by O-phenanthroline and MGTA and activated by cobalt. The amino acid sequence of CPM indicated that the C-terminal hydrophobic region might be a signal for membrane attachment via a glycosylphosphatidylinositol (GPI) anchor. CPM is involved in peptide metabolism on both the cell surface and in extracellular fluids. CPM functions not only as a protease but also as a binding partner in cell-surface protein-protein interactions.
Molecular Weight 47.1 kDa
UniProt P14384
Research Area Signaling, Innate Immunity
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM MES, 100 mM NaCl, pH 6.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Carboxypeptidase M (CPM) (AA 18-422) (Active) protein (His tag) (ABIN2180904) Human Carboxypeptidase M, His Tag on SDS-PAGE under reducing (R) condition. The gel w...
Background publications Tan, Balsitis, Black, Blöchl, Mao, Becker, Schacht, Skidgel: "Effect of mutation of two critical glutamic acid residues on the activity and stability of human carboxypeptidase M and characterization of its signal for glycosylphosphatidylinositol anchoring." in: The Biochemical journal, Vol. 370, Issue Pt 2, pp. 567-78, 2003 (PubMed).

Tan, Chan, Steiner, Schilling, Skidgel: "Molecular cloning and sequencing of the cDNA for human membrane-bound carboxypeptidase M. Comparison with carboxypeptidases A, B, H, and N." in: The Journal of biological chemistry, Vol. 264, Issue 22, pp. 13165-70, 1989 (PubMed).