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  • 产品名称：CD244Molecule,NaturalKillerCellReceptor2B4(CD244)(AA22-221)(Active)protein(Hist

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  • 产品厂商：ACROBiosystems
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简单介绍：
CD244Molecule,NaturalKillerCellReceptor2B4(CD244)(AA22-221)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 23.1 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA.Immobilized rh CD244 / 2B4 at 2 μg/mL ( 100 μL/well ) can bind human CD48. The EC50 of human CD48 is 0.39 μg/mL.

SDS-PAGE: due to glycosylation.
Background Natural killer cell receptor 2B4 is also known as NK cell type I receptor protein 2B4 (NKR2B4 or h2B4), SLAM family member 4 (SLAMF4), Signaling lymphocytic activation molecule 4, CD antigen CD244. NKR2B4 / CD244 contains two Ig-like (immunoglobulin-like) domains. CD244 is expressed in spleen, PBL, followed by lung, liver, testis and small intestine. CD244 interacts with CD48. Following phosphorylation, CD244 is able to recruit PTPN11/SHP-2 and SH2D1A/SAP. SLAMF4 modulate other receptor-ligand interactions to enhance leukocyte activation. CD244/2B4 is the only heterophilic receptor of SLAM family.
Molecular Weight 23.1 kDa
NCBI Accession NP_057466
UniProt Q9BZW8
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for CD244 Molecule, Natural Killer Cell Receptor 2B4 (CD244) (AA 22-221) (Active) protein (His tag) (ABIN2180730) Human 2B4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained over...
Background publications Nakajima, Cella, Langen, Friedlein, Colonna: "Activating interactions in human NK cell recognition: the role of 2B4-CD48." in: European journal of immunology, Vol. 29, Issue 5, pp. 1676-83, 1999 (PubMed).

Griffith, Wiley, Kubin, Sedger, Maliszewski, Fanger: "Monocyte-mediated tumoricidal activity via the tumor necrosis factor-related cytokine, TRAIL." in: The Journal of experimental medicine, Vol. 189, Issue 8, pp. 1343-54, 1999 (PubMed).

Kubin, Parshley, Din, Waugh, Davis-Smith, Smith, Macduff, Armitage, Chin, Cassiano, Borges, Petersen, Trinchieri, Goodwin: "Molecular cloning and biological characterization of NK cell activation-inducing ligand, a counterstructure for CD48." in: European journal of immunology, Vol. 29, Issue 11, pp. 3466-77, 1999 (PubMed).

Boles, Nakajima, Colonna, Chuang, Stepp, Bennett, Kumar, Mathew: "Molecular characterization of a novel human natural killer cell receptor homologous to mouse 2B4." in: Tissue antigens, Vol. 54, Issue 1, pp. 27-34, 1999 (PubMed).