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  • 产品名称：CarbonicAnhydraseIV(CA4)(AA19-283)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CarbonicAnhydraseIV(CA4)(AA19-283)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 31 kDa. The protein migrates as 33 kDa under reducing (R) condition (SDS-PAGE).
Purity >92 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its esterase activity. The specific activity is >2 pmol/min/μg.
Background Carbonic anhydrase 4 (CA4) is also known as Carbonate dehydratase IV, Carbonic anhydrase IV, which belongs to the alpha-carbonic anhydrase family. CA4 may stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid. The enzyme regulation is activated by histamine, L-adrenaline, D-phenylalanine, L- and D-histidine. CA4 can interacts with SLC4A4.
Molecular Weight 31 kDa
UniProt P22748
Research Area Neurology
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 150 mM NaCl, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Carbonic Anhydrase IV (CA4) (AA 19-283) (Active) protein (His tag) (ABIN2180671) Human Carbonic Anhydrase IV, His Tag on SDS-PAGE under reducing (R) condition. The ge...
Background publications Di Fiore, Monti, Hilvo, Parkkila, Romano, Scaloni, Pedone, Scozzafava, Supuran, De Simone: "Crystal structure of human carbonic anhydrase XIII and its complex with the inhibitor acetazolamide." in: Proteins, Vol. 74, Issue 1, pp. 164-75, 2008 (PubMed).

Yang, Alvarez, Chakarova, Jiang, Karan, Frederick, Zhao, Sauvé, Li, Zrenner, Wissinger, Hollander, Katz, Baehr, Cremers, Casey, Bhattacharya, Zhang: "Mutant carbonic anhydrase 4 impairs pH regulation and causes retinal photoreceptor degeneration." in: Human molecular genetics, Vol. 14, Issue 2, pp. 255-65, 2004 (PubMed).

Zhu, Paul, Vaughn, Morales: "Characterization and reactivity of monoclonal antibodies to the Miller strain of transmissible gastroenteritis virus of swine." in: American journal of veterinary research, Vol. 51, Issue 2, pp. 232-8, 1990 (PubMed).

Zhu, Sly: "Carbonic anhydrase IV from human lung. Purification, characterization, and comparison with membrane carbonic anhydrase from human kidney." in: The Journal of biological chemistry, Vol. 265, Issue 15, pp. 8795-801, 1990 (PubMed).