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  • 产品名称：TissueFactorPathwayInhibitor2(TFPI2)(AA23-213)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
TissueFactorPathwayInhibitor2(TFPI2)(AA23-213)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 22.7 kDa. The protein migrates as 26-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca - RPKPVE - Nval - WRK (Dnp) - NH2. The IC50 value is < 2.5 nM, as measured in 100μL reaction mixture containing 1.25 ng trypsin, 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15M NaCl, 0.05% Brij-35, pH 7.5.
Background Tissue Factor Pathway Inhibitor 2 (TFPI-2) is also known as placental protein 5 (PP5) and retinal pigment epithelial cell factor 1 (REF-1), is a member of the Kunitz-type serine proteinase inhibitor family, is a structural homologue of tissue factor pathway inhibitor (TFPI). TFPI2 is highly abundant in the full-term placenta and widely expressed in umbilical vein endothelial cells, liver, placenta, heart, pancreas, and maternal serum at advanced pregnancy. TFPI-2 may play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa and has no effect on thrombin. Reduced synthesis of TFPI-2 has been related to numerous pathophysiological processes such as inflammation, angiogenesis, atherosclerosis, retinal degeneration and tumor growth / metastasis.
Molecular Weight 22.7 kDa
NCBI Accession NP_006519
UniProt P48307
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Tissue Factor Pathway Inhibitor 2 (TFPI2) (AA 23-213) (Active) protein (His tag) (ABIN2181806) Human TFPI-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained o...
Background publications Rao, Liu, Peavey, Woodley: "Novel extracellular matrix-associated serine proteinase inhibitors from human skin fibroblasts." in: Archives of biochemistry and biophysics, Vol. 317, Issue 1, pp. 311-4, 1995 (PubMed).