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  • 产品名称：InsulinReceptor(INSR)(AA28-944)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
InsulinReceptor(INSR)(AA28-944)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 86.8 kDa (alpha subunit) & 23 kDa (beta subunit). The protein migrates as 120-135 kDa (alpha subunit) & 35-46 kDa (beta subunit) under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Insulin receptor (INSR) is also known as CD antigen CD220, which can be cleaved into the following 2 chains: Insulin receptor subunit alpha and Insulin receptor subunit beta. INSR is a tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains carry the insulin-binding regions, while the beta chains carry the kinase domain. Forms a hybrid receptor with IGF1R, the hybrid is a tetramer consisting of 1 alpha chain and 1 beta chain of INSR and 1 alpha chain and 1 beta chain of IGF1R. In addition to binding insulin, the insulin receptor can bind insulin-like growth factors (IGFI and IGFII). Isoform Short of INSR has a higher affinity for IGFII binding. When present in a hybrid receptor with IGF1R, INSR binds IGF1.
Molecular Weight 86.8 kDa (alpha subunit) & 23 kDa (beta subunit)
NCBI Accession NP_000199
UniProt P06213
Pathways NF-kappaB Signaling, RTK Signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Insulin Receptor (INSR) (AA 28-944) (Active) protein (His tag) (ABIN2181405) Human Insulin R (aa 28-944), His Tag on SDS-PAGE under reducing (R) condition. The ge...
Background publications Menting, Whittaker, Margetts, Whittaker, Kong, Smith, Watson, Záková, Kletvíková, Jirá?ek, Chan, Steiner, Dodson, Brzozowski, Weiss, Ward, Lawrence: "How insulin engages its primary on the insulin receptor." in: Nature, Vol. 493, Issue 7431, pp. 241-5, 2013 (PubMed).

Slaaby, Schäffer, Lautrup-Larsen, Andersen, Shaw, Mathiasen, Brandt: "Hybrid receptors formed by insulin receptor (IR) and insulin-like growth factor I receptor (IGF-IR) have low insulin and high IGF-1 affinity irrespective of the IR splice variant." in: The Journal of biological chemistry, Vol. 281, Issue 36, pp. 25869-74, 2006 (PubMed).

Pandini, Frasca, Mineo, Sciacca, Vigneri, Belfiore: "Insulin/insulin-like growth factor I hybrid receptors have different biological characteristics depending on the insulin receptor isoform involved." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 39684-95, 2002 (PubMed).

Kadowaki, Bevins, Cama, Ojamaa, Marcus-Samuels, Kadowaki, Beitz, McKeon, Taylor: "Two mutant alleles of the insulin receptor gene in a patient with extreme insulin resistance." in: Science (New York, N.Y.), Vol. 240, Issue 4853, pp. 787-90, 1988 (PubMed).