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  • 产品名称：serpinPeptidaseInhibitor,CladeF(Alpha-2Antiplasmin,PigmentEpitheliumDerivedFact

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  • 产品厂商：ACROBiosystems
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简单介绍：
serpinPeptidaseInhibitor,CladeF(Alpha-2Antiplasmin,PigmentEpitheliumDerivedFactor),Member1(SERPINF1)(AA20-418)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 45.2 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to enhance the adhesion of Saos-2 human osteosarcoma cells to bovine Collagen I coated plate. The ED50 for this effect is typically 0.5-1.6 μg/mL.
Background Erpin F1 (SERPINF1) is also known as Pigment epithelium-derived factor (PEDF), Cell proliferation-inducing gene 35 protein (PIG35). Serpin F1 belongs to the serpin family. Serpin F1 is expressed in quiescent cells. PEDF has a variety of functions including antiangiogenic, antitumorigenic, and neurotrophic properties. Endothelial cell migration is inhibited by SERPINF1/ PEDF. PEDF / SERPINF1 suppresses retinal neovascularization and endothelial cell proliferation. PEDF is also responsible for apoptosis of endothelial cells either through the p38 MAPK pathway or through the FAS/FASL pathway. PEDF also displays neurotrophic functions.
Molecular Weight 45.2 kDa
NCBI Accession NP_002606
UniProt P36955
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1 (SERPINF1) (AA 20-418) (Active) protein (His tag) (ABIN2181751) Human Serpin F1, His Tag on SDS-PAGE under reducing (R) condition. The gel was staine...
Background publications Amaral, Becerra: "Effects of human recombinant PEDF protein and PEDF-derived peptide 34-mer on choroidal neovascularization." in: Investigative ophthalmology & visual science, Vol. 51, Issue 3, pp. 1318-26, 2010 (PubMed).

Duh, Yang, Suzuma, Miyagi, Youngman, Mori, Katai, Yan, Suzuma, West, Davarya, Tong, Gehlbach, Pearlman, Crabb, Aiello, Campochiaro, Zack: "Pigment epithelium-derived factor suppresses ischemia-induced retinal neovascularization and VEGF-induced migration and growth." in: Investigative ophthalmology & visual science, Vol. 43, Issue 3, pp. 821-9, 2002 (PubMed).