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  • 产品名称：Fms-RelatedtyrosineKinase1(VascularEndothelialGrowthFactor/vascularPermeability

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  • 产品厂商：ACROBiosystems
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简单介绍：
Fms-RelatedtyrosineKinase1(VascularEndothelialGrowthFactor/vascularPermeabilityFactorReceptor)(FLT1)(AA27-756)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 83 kDa. The protein migrates as 115-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >98 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to inhibit the VEGF-dependent proliferation of HUVEC human umbilical vein endothelial cells. The ED50 for this effect is typically 2-25 ng/mL. Measured by its ability to bind human VEGF165 (Cat# VE5-H4210) in the SPR assay (Biacore 2000) with an estimated KD of 0.1nM.

SDS-PAGE: due to glycosylation.
Background Vascular endothelial growth factor receptor 1 (VEGFR1) is also known as Fms-like tyrosine kinase 1 (FLT-1), Tyrosine-protein kinase receptor FLT, is a single-pass type I membrane protein and secreted protein which belongs to the protein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily. VEGFR1 is detected in normal lung, but also in placenta, liver, kidney, heart and brain tissues and specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. VEGFR1 acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. VEGFR1 may play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. VEGFR1 can promote endothelial cell proliferation, survival and angiogenesis in *****hood.
Molecular Weight 83 kDa
NCBI Accession NP_002010
UniProt P17948
Pathways RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) (AA 27-756) (Active) protein (His tag) (ABIN2181915) Human VEGF R1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ...
Binding Studies (Bind) image for Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) (AA 27-756) (Active) protein (His tag) (ABIN2181915) Immobilized Human VEGF165 with a linear range of 3-50 ng/mL.
Background publications Iyer, Darley, Acharya: "Structural insights into the binding of vascular endothelial growth factor-B by VEGFR-1(D2): recognition and specificity." in: The Journal of biological chemistry, Vol. 285, Issue 31, pp. 23779-89, 2010 (PubMed).

Barleon, Sozzani, Zhou, Weich, Mantovani, Marmé: "Migration of human monocytes in response to vascular endothelial growth factor (VEGF) is mediated via the VEGF receptor flt-1." in: Blood, Vol. 87, Issue 8, pp. 3336-43, 1996 (PubMed).

Seetharam, Gotoh, Maru, Neufeld, Yamaguchi, Shibuya: "A unique signal transduction from FLT tyrosine kinase, a receptor for vascular endothelial growth factor VEGF." in: Oncogene, Vol. 10, Issue 1, pp. 135-47, 1995 (PubMed).

Kendall, Thomas: "Inhibition of vascular endothelial cell growth factor activity by an endogenously encoded soluble receptor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 22, pp. 10705-9, 1993 (PubMed).

Shibuya, Yamaguchi, Yamane, Ikeda, Tojo, Matsushime, Sato: "Nucleotide sequence and expression of a novel human receptor-type tyrosine kinase gene (flt) closely related to the fms family." in: Oncogene, Vol. 5, Issue 4, pp. 519-24, 1990 (PubMed).