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  • 产品名称：CystatinF(Leukocystatin)(CST7)(AA20-145)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CystatinF(Leukocystatin)(CST7)(AA20-145)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.6 kDa. The protein migrates as 20-24 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC. The IC50 value is <7.0 nm.="" <="" td="">
Background Cystatin-7 (CST7) is also known as Cystatin-F, Cystatin-like metastasis-associated protein (CMAP), Leukocystatin, which is a disulfide-linked homodimer belongs to the cystatin family. CST7 is primarily expressed in peripheral blood cells and spleen. CST7 inhibits papain and cathepsin L but with affinities lower than other cystatins. CST7 may play a role in immune regulation through inhibition of a unique target in the hematopoietic system.
Molecular Weight 15.6 kDa
UniProt O76096
Research Area Apoptosis/Necrosis, Angiogenesis, Serum/Plasma Proteins
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Cystatin F (Leukocystatin) (CST7) (AA 20-145) (Active) protein (His tag) (ABIN2180924) Human Cystatin F, His Tag on SDS-PAGE under reducing (R) condition. The gel was stain...
Background publications Schüttelkopf, Hamilton, Watts, van Aalten: "Structural basis of reduction-dependent activation of human cystatin F." in: The Journal of biological chemistry, Vol. 281, Issue 24, pp. 16570-5, 2006 (PubMed).

Nathanson, Wassélius, Wallin, Abrahamson: "Regulated expression and intracellular localization of cystatin F in human U937 cells." in: European journal of biochemistry / FEBS, Vol. 269, Issue 22, pp. 5502-11, 2002 (PubMed).