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  • 产品名称:GM-CSF(AA18-144)(Active)Protein

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  • 产品厂商:ACROBiosystems
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简单介绍:
GM-CSF(AA18-144)(Active)Protein
详情介绍:
Brand ActiveMax®
Characteristics This protein carries no "tag". The protein has a calculated MW of 14.5 kDa. The protein migrates as 16-26 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 0.1 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: The bio-activity was determined by dose-dependent stimulation of the proliferation of TF-1 cells. The ED50<25 pg="" ml,="" corresponding="" to="" a="" specific="" activity="" of="">4E+7 Unit/mg.

SDS-PAGE: due to glycosylation.
Background Granulocyte-macrophage colony-stimulating factor (GM-CSF) is also known as Colony stimulating factor 2 (granulocyte-macrophage), is a cytokine initially characterized by its ability to induce colonies of granulocytes and macrophages from myeloid progenitor cells, and is secreted by macrophages, T cells, mast cells, endothelial cells and fibroblasts. GM-CSF is a cytokine that functions as a white blood cell growth factor. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. Monocytes exitthe circulation and migrate into tissue, whereupon they mature into macrophages and dendritic cells. Thus, it is part of the immune/inflammatory cascade, by which activation of a small number of macrophages can rapidly lead to an increase in their numbers, a process crucial for fighting infection. The active form of the protein is found extracellularly as a homodimer. Human GM-CSF glycosylated in its mature form. As a part of the immune/inflammatory cascade, GM-CSF promotes Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity, and thus worthy of consideration for therapeutic target. GM-CSF has also recently been evaluated in clinical trials for its potential as a vaccine adjuvant in HIV-infected patients. The preliminary results have been promising. GM-CSF is also used as a medication to stimulate the production of white blood cells following chemotherapy.
Molecular Weight 14.5 kDa
NCBI Accession NP_000749
UniProt P04141
Research Area Immunology, Innate Immunity, Cytokines
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for GM-CSF (AA 18-144) (Active) Protein (ABIN2181169) Human GM-CSF on SDS-PAGE under reducing (R) condition. The gel was stained overnight ...
Background publications Ait-ghezala, Abdullah, Volmar, Paris, Luis, Quadros, Mouzon, Mullan, Keegan, Parrish, Crawford, Mathura, Mullan: "Diagnostic utility of APOE, soluble CD40, CD40L, and Abeta1-40 levels in plasma in Alzheimer's disease." in: Cytokine, Vol. 44, Issue 2, pp. 283-7, 2008 (PubMed).