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  • 产品名称：ProproteinConvertaseSubtilisin/kexinType9(PCSK9)(AA35-694)(Active)protein(Hista

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  • 产品厂商：ACROBiosystems
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简单介绍：
ProproteinConvertaseSubtilisin/kexinType9(PCSK9)(AA35-694)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 72 kDa. The protein migrates as 20 kDa and 64 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation and proteolytic digestion.
Purity >97 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA Immobilized mouse PCSK9 at 10 μg/mL (100 μL/well) can bind biotinylated rhLDLR. The EC50 of biotinylated human LDLR is 0.1 μg/mL.
Background Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).
Molecular Weight 72 kDa
UniProt Q80W65
Research Area Proteolysis / Ubiquitin, Metabolism, Amino Acids
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) (AA 35-694) (Active) protein (His tag) (ABIN2181589) Mouse PCSK9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ov...
Binding Studies (Bind) image for Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) (AA 35-694) (Active) protein (His tag) (ABIN2181589) Immobilized Human LDL R, Strep Tag (Cat# LDR-H5281) at 10 μg/mL (100 μl/well) can bin...
Background publications Abifadel, Varret, Rabès, Allard, Ouguerram, Devillers, Cruaud, Benjannet, Wickham, Erlich, Derré, Villéger, Farnier, Beucler, Bruckert, Chambaz, Chanu, Lecerf, Luc, Moulin, Weissenbach, Prat, Krempf et al.: "Mutations in PCSK9 cause autosomal dominant hypercholesterolemia. ..." in: Nature genetics, Vol. 34, Issue 2, pp. 154-6, 2003 (PubMed).

Seidah, Benjannet, Wickham, Marcinkiewicz, Jasmin, Stifani, Basak, Prat, Chretien: "The secretory proprotein convertase neural apoptosis-regulated convertase 1 (NARC-1): liver regeneration and neuronal differentiation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 3, pp. 928-33, 2003 (PubMed).