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  • 产品名称：CD69(CD69)(AA62-199)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CD69(CD69)(AA62-199)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.8 kDa. The protein migrates as 23 kDa and 26 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
Purity >92 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Early activation antigen CD69 is also known as C-type lectin domain family 2 member C (CLEC2C), Activation inducer molecule (AIM), Early T-cell activation antigen p60, Leukocyte surface antigen Leu-23. CD69 contains one C-type lectin domain. CD69 / CLEC2C is expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets. CD69 is induced by antigens, mitogens or activators of PKC on the surface of T and B-lymphocytes and by interaction of IL-2 with the p75 IL-2R on the surface of NK cells. CD69 / CLEC2C involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.
Molecular Weight 16.8 kDa
UniProt Q07108
Research Area CD Antigens, Surface Receptors of Immune Cells, Immunology
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for CD69 (CD69) (AA 62-199) (Active) protein (His tag) (ABIN2180825) Human CD69, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ove...
Background publications Vanek, Nálezková, Kavan, Borovicková, Pompach, Novák, Kumar, Vannucci, Hudecek, Hofbauerová, Kopecký, Brynda, Kolenko, Dohnálek, Kaderávek, Chmelík, Gorcík, Zídek, Sklenár, Bezouska: "Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemical stability display prolonged circulation and remain intact in the blood of mice." in: The FEBS journal, Vol. 275, Issue 22, pp. 5589-606, 2008 (PubMed).

Natarajan, Sawicki, Margulies, Mariuzza: "Crystal structure of human CD69: a C-type lectin-like activation marker of hematopoietic cells." in: Biochemistry, Vol. 39, Issue 48, pp. 14779-86, 2001 (PubMed).

Llera, Viedma, Sánchez-Madrid, Tormo: "Crystal structure of the C-type lectin-like domain from the human hematopoietic cell receptor CD69." in: The Journal of biological chemistry, Vol. 276, Issue 10, pp. 7312-9, 2001 (PubMed).