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  • 产品名称：CD274Molecule(CD274)(AA19-238)(Active)protein(FcTag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CD274Molecule(CD274)(AA19-238)(Active)protein(FcTag)
详情介绍：
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 51.4 kDa. The protein migrates as 65-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized mouse PDL1 protein Fc Chimera (Catalog # PD1-M5251) at 5 μg/mL can bind mouse PDCD1 protein (Catalog # PD1-M5228) with a linear range of 1-20 μg/mL .

SDS-PAGE: due to glycosylation.
Background Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.
Molecular Weight 51.4 kDa
NCBI Accession NP_068693
UniProt Q9EP73
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for CD274 Molecule (CD274) (AA 19-238) (Active) protein (Fc Tag) (ABIN2181638) Mouse PD-L1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained ove...
Binding Studies (Bind) image for CD274 Molecule (CD274) (AA 19-238) (Active) protein (Fc Tag) (ABIN2181638) Immobilized Mouse PD-L1 / B7-H1 Protein, Fc Tag (Cat# PD1-M5251) at 10μg/mL (100μL/we...
Background publications Freeman, Long, Iwai, Bourque, Chernova, Nishimura, Fitz, Malenkovich, Okazaki, Byrne, Horton, Fouser, Carter, Ling, Bowman, Carreno, Collins, Wood, Honjo: "Engagement of the PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation." in: The Journal of experimental medicine, Vol. 192, Issue 7, pp. 1027-34, 2000 (PubMed).

Dong, Zhu, Tamada, Chen: "B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion." in: Nature medicine, Vol. 5, Issue 12, pp. 1365-9, 1999 (PubMed).