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  • 产品名称：InducibleT-CellCo-StimulatorLigand(ICOSLG)(AA19-258)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
InducibleT-CellCo-StimulatorLigand(ICOSLG)(AA19-258)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 27.5 kDa. The protein migrates as 35-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized human ICOSLG / B7H2 at 1 μg/mL ( 100 μL/well ) can bind human ICOS with a linear range of 3.2 - 400 ng/mL.

SDS-PAGE: due to glycosylation.
Background ICOS ligand (ICOSLG) is also known as B7 homolog 2 (B7-H2), B7-related protein 1 (B7RP-1) and CD antigen CD275, which belongs to the immunoglobulin superfamily and BTN/MOG family. ICOSLG contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. Isoform 1 is widely expressed, while isoform 2 is detected only in lymph nodes, leukocytes and spleen. B7-H2 is ligand for the T-cell-specific cell surface receptor ICOS. B7-H2 acts as a costimulatory signal for T-cell proliferation and cytokine secretion and induces also B-cell proliferation and differentiation into plasma cells. B7-H2 could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.
Molecular Weight 27.5 kDa
NCBI Accession NP_056074
UniProt O75144
Pathways Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Inducible T-Cell Co-Stimulator Ligand (ICOSLG) (AA 19-258) (Active) protein (His tag) (ABIN2181242) Human B7-H2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ov...
Binding Studies (Bind) image for Inducible T-Cell Co-Stimulator Ligand (ICOSLG) (AA 19-258) (Active) protein (His tag) (ABIN2181242) Immobilized Human B7-H2, His Tag (Cat# B72-H5221) at 1μg/mL (100 µl/well),can bind Hu...
Background publications Aicher, Hayden-Ledbetter, Brady, Pezzutto, Richter, Magaletti, Buckwalter, Ledbetter, Clark: "Characterization of human inducible costimulator ligand expression and function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 9, pp. 4689-96, 2000 (PubMed).