产品详情
  • 产品名称:Interleukin2Receptor,alpha(IL2RA)(AA22-213)(Active)protein(FcTag)

  • 产品型号:
  • 产品厂商:ACROBiosystems
  • 产品文档:
你添加了1件商品 查看购物车
简单介绍:
Interleukin2Receptor,alpha(IL2RA)(AA22-213)(Active)protein(FcTag)
详情介绍:
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 48.4 Kda. The protein migrates as 60-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Interleukin-2 receptor subunit alpha (IL2RA) is also known as IL-2R subunit alpha, IL-2-RA, IL2-RA, TAC antigen, p55, CD antigen CD25, is a type I transmembrane glycoprotein. IL2RA is expressed on activated T cells and regulatory T cells, and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covelently with the IL2 receptor beta and gamma chain, and subsequently initiates the intacellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling.
Molecular Weight 48.4 Kda
NCBI Accession NP_000408
UniProt P01589
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Interleukin 2 Receptor, alpha (IL2RA) (AA 22-213) (Active) protein (Fc Tag) (ABIN2181381) Interleukin 2 Receptor, alpha (IL2RA) (AA 22-213) (Active) protein (Fc Tag)
Background publications Wang, Rickert, Garcia: "Structure of the quaternary complex of interleukin-2 with its alpha, beta, and gammac receptors." in: Science (New York, N.Y.), Vol. 310, Issue 5751, pp. 1159-63, 2005 (PubMed).

Miedel, Hulmes, Weber, Bailon, Pan: "Structural analysis of recombinant soluble human interleukin-2 receptor. Primary structure, assignment of disulfide bonds and core IL-2 binding structure." in: Biochemical and biophysical research communications, Vol. 154, Issue 1, pp. 372-9, 1988 (PubMed).

Leonard, Depper, Kanehisa, Krönke, Peffer, Svetlik, Sullivan, Greene: "Structure of the human interleukin-2 receptor gene." in: Science (New York, N.Y.), Vol. 230, Issue 4726, pp. 633-9, 1985 (PubMed).