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  • 产品名称：ProproteinConvertaseSubtilisin/kexinType9(PCSK9)(AA31-692),(Asp374Tyr-Mutation)

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  • 产品厂商：ACROBiosystems
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简单介绍：
ProproteinConvertaseSubtilisin/kexinType9(PCSK9)(AA31-692),(Asp374Tyr-Mutation)(Active)protein(Histag)
详情介绍：
Characteristics Human PCSK9 (D374Y) Protein is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 72 kDa. The predicted N-terminus is Gln 31. The reducing (R) protein migrates as 18 kDa and 65 kDa in SDS-PAGE due to glycosylation and proteolytic digestion.The D374Y mutation results in higher affinity of PCSK9 for LDLR.
Purity >92 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).Especially,the D374Y mutation results in higher affinity of PCSK9 for LDLR.
Molecular Weight 72 kDa
UniProt Q8NBP7
Research Area Proteolysis / Ubiquitin, Metabolism, Amino Acids
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) (AA 31-692), (Asp374Tyr-Mutation) (Active) protein (His tag) (ABIN2181588) Human PCSK9 (D374Y), His Tag on SDS-PAGE under reducing (R) condition. The gel was st...
Binding Studies (Bind) image for Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) (AA 31-692), (Asp374Tyr-Mutation) (Active) protein (His tag) (ABIN2181588) Immobilized Human LDL R, His Tag (Cat# LDR-H5224) at 10 μg/mL (100 μl/well) can bind ...
Background publications Abifadel, Varret, Rabès, Allard, Ouguerram, Devillers, Cruaud, Benjannet, Wickham, Erlich, Derré, Villéger, Farnier, Beucler, Bruckert, Chambaz, Chanu, Lecerf, Luc, Moulin, Weissenbach, Prat, Krempf et al.: "Mutations in PCSK9 cause autosomal dominant hypercholesterolemia. ..." in: Nature genetics, Vol. 34, Issue 2, pp. 154-6, 2003 (PubMed).

Seidah, Benjannet, Wickham, Marcinkiewicz, Jasmin, Stifani, Basak, Prat, Chretien: "The secretory proprotein convertase neural apoptosis-regulated convertase 1 (NARC-1): liver regeneration and neuronal differentiation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 3, pp. 928-33, 2003 (PubMed).