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  • 产品名称：LeukocyteImmunoglobulin-LikeReceptor,SubfamilyB(WithTMandITIMDomains),Member2(L

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  • 产品厂商：ACROBiosystems
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简单介绍：
LeukocyteImmunoglobulin-LikeReceptor,SubfamilyB(WithTMandITIMDomains),Member2(LILRB2)(AA22-461)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 48.6 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >92 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability of the immobilized protein to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 80000 cells/well are added to LILRB2 coated plates (8.8 μg/mL with 100 μL/well), > 40% cells will adhere for 1 hour incubation at 37°C.

SDS-PAGE: due to glycosylation.
Background Leukocyte immunoglobulin-like receptor subfamily B member 2 (LILRB2) is also known as CD85 antigen-like family member D (CD85d), Immunoglobulin-like transcript 4 (ILT-4), Monocyte / macrophage immunoglobulin-like receptor 10 (MIR-10), which is a member of the the subfamily B class of LIR receptors. LILRB2 is receptor for class I MHC antigens. LILRB2 recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. LILRB2 competes with CD8A for binding to class I MHC antigens. LILRB2 / CD85d inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
Molecular Weight 48.6 kDa
UniProt Q8N423
Research Area Immunology, Adaptive Immunity
Pathways Cellular Response to Molecule of Bacterial Origin
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 2 (LILRB2) (AA 22-461) (Active) protein (His tag) (ABIN2181472) Human LILRB2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained o...
Background publications Shiroishi, Tsumoto, Amano, Shirakihara, Colonna, Braud, Allan, Makadzange, Rowland-Jones, Willcox, Jones, van der Merwe, Kumagai, Maenaka: "Human inhibitory receptors Ig-like transcript 2 (ILT2) and ILT4 compete with CD8 for MHC class I binding and bind preferentially to HLA-G." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 15, pp. 8856-61, 2003 (PubMed).

Borges, Hsu, Fanger, Kubin, Cosman: "A family of human lymphoid and myeloid Ig-like receptors, some of which bind to MHC class I molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 11, pp. 5192-6, 1998 (PubMed).

Fanger, Cosman, Peterson, Braddy, Maliszewski, Borges: "The MHC class I binding proteins LIR-1 and LIR-2 inhibit Fc receptor-mediated signaling in monocytes." in: European journal of immunology, Vol. 28, Issue 11, pp. 3423-34, 1998 (PubMed).