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  • 产品名称：CarcinoembryonicAntigen-RelatedCellAdhesionMolecule6(Non-SpecificCrossReactingA

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  • 产品厂商：ACROBiosystems
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简单介绍：
CarcinoembryonicAntigen-RelatedCellAdhesionMolecule6(Non-SpecificCrossReactingAntigen)(CEACAM6)(AA35-320)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 32 kDa. The protein migrates as 35-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by the ability of the immobilized protein to support the adhesion of calcium ionophore treated human neutrophils. When 2 x 105 cells/well are added to CEACAM6 coated plates (10 μg/mL, 100 μL/well), 45-70% of the cells will adhere after 20 minutes at 37oC.

SDS-PAGE: resulting from glycosylation.
Background Carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific cross reacting antigen) (CEACAM6) is also known as CD66c (Cluster of Differentiation 66c), CEAL, NCA, and is one of seven human CEACAM family members within the immunoglobulin superfamily. In humans, CEACAMs include type I transmembrane proteins (CEACAM1, CEACAM3, and CEACAM4) and GPI-linked molecules (CEACAM5 through CEACAM8). There is no human CEACAM2. CEACAM 6 contains one N-terminal V-type Ig-like domain (N domain), followed by two C2-type Ig-like domains. It shows considerable glycosylation, including (sialyl) LewisX, which mediates binding to E-selectin, galectins and some bacterial fimbrae. CEACAM-6 is expressed by granulocytes and their progenitors. It is also expressed by epithelia of various organs and is upregulated in pancreatic and colon adenocarcinomas, as well as hyperplastic polyps. Resistance to adhesion-related apoptosis in tumor cells is conferred in the condition of CEACAM6 overexpression.
Molecular Weight 32 kDa
NCBI Accession NP_002474
UniProt P40199
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (Non-Specific Cross Reacting Antigen) (CEACAM6) (AA 35-320) (Active) protein (His tag) (ABIN2180869) Human CEACAM-6, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained...
Background publications Duxbury, Ito, Ashley, Whang: "CEACAM6 cross-linking induces caveolin-1-dependent, Src-mediated focal adhesion kinase phosphorylation in BxPC3 pancreatic adenocarcinoma cells." in: The Journal of biological chemistry, Vol. 279, Issue 22, pp. 23176-82, 2004 (PubMed).

Beauchemin, Draber, Dveksler, Gold, Gray-Owen, Grunert, Hammarström, Holmes, Karlsson, Kuroki, Lin, Lucka, Najjar, Neumaier, Obrink, Shively, Skubitz, Stanners, Thomas, Thompson, Virji, von Kleist et al.: "Redefined nomenclature for members of the carcinoembryonic antigen family. ..." in: Experimental cell research, Vol. 252, Issue 2, pp. 243-9, 2001 (PubMed).

Schölzel, Zimmermann, Schwarzkopf, Grunert, Rogaczewski, Thompson: "Carcinoembryonic antigen family members CEACAM6 and CEACAM7 are differentially expressed in normal tissues and oppositely deregulated in hyperplastic colorectal polyps and early adenomas." in: The American journal of pathology, Vol. 156, Issue 2, pp. 595-605, 2000 (PubMed).