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  • 产品名称：TumorNecrosisFactorReceptorSuperfamily,Member1B(TNFRSF1B)(AA23-257)(Active)prot

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  • 产品厂商：ACROBiosystems
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简单介绍：
TumorNecrosisFactorReceptorSuperfamily,Member1B(TNFRSF1B)(AA23-257)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26 kDa. The protein migrates as 35-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized human TNFa (Catalog # TNA-H4211) at 5 μg/mL (100 μL/well) can bind human TNFR2 (Catalog # TN2-H5227). The EC50 of human TNFR2 is 647 ng/mL.

SDS-PAGE: due to glycosylation.
Background TNF RI is also known as the p60 or p55 TNFR) and TNF RII (the p75 or p80 TNFR) are two distinct type I transmembrane glycoproteins that bind TNF with highaffinity.Both RI and RII are prototypic members of the TNF receptor superfamily and have been designated TNFRSF1A and TNFRSF1B, respectively. Human TNF RII cDNA encodes a 461 amino acid (aa) residue precursor protein with a 22 aa putative signal peptide, a 235 aa extracellular domain, a 20 aa transmembrane domain and a 174 aa cytoplasmic domain. TNFRII is expressed in fetal brain. The protein is produced naturally as a soluble form (sTNFRII). The soluble receptor inhibits TNFα action by competing with cell surface receptors in binding TNFα, thereby blocking its biologic effects. TNFRII is strongly expressed at the cartilage-pannus junction, and plays a major role in a subset of families with multiple cases of rheumatoid arthritis (RA). Further, high plasma levels of sTNFRII were significantly associated with increased incidence of coronary heart disease, independent of established cardiovascular risk factors, and seems to be useful for monitoring the inflammatory activity of sarcoidosis.
Molecular Weight 26 kDa
NCBI Accession NP_001057
UniProt P20333
Pathways NF-kappaB Signaling, Apoptosis, Cellular Response to Molecule of Bacterial Origin, Hepatitis C
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) (AA 23-257) (Active) protein (His tag) (ABIN2181842) Human TNFR2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ov...
Binding Studies (Bind) image for Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) (AA 23-257) (Active) protein (His tag) (ABIN2181842) Immobilized Human TNF-beta (Cat# TNB-H5214) at 5 μg/mL (100 µl/well),can bind Human ...
Binding Studies (Bind) image for Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) (AA 23-257) (Active) protein (His tag) (ABIN2181842) Immobilized Human TNF-alpha . The EC50 of human TNFR2 is 647 ng/mL.
Background publications Chung, Guan, Yuan, Albina, Chin: "Ankyrin repeat and SOCS box 3 (ASB3) mediates ubiquitination and degradation of tumor necrosis factor receptor II." in: Molecular and cellular biology, Vol. 25, Issue 11, pp. 4716-26, 2005 (PubMed).

Shai, Schulze, Manson, Rexrode, Stampfer, Mantzoros, Hu: "A prospective study of soluble tumor necrosis factor-alpha receptor II (sTNF-RII) and risk of coronary heart disease among women with type 2 diabetes." in: Diabetes Care, Vol. 28, Issue 6, pp. 1376-82, 2005 (PubMed).

Ziegenhagen, Fitschen, Martinet, Schlaak, Müller-Quernheim: "Serum level of soluble tumour necrosis factor receptor II (75 kDa) indicates inflammatory activity of sarcoidosis." in: Journal of internal medicine, Vol. 248, Issue 1, pp. 33-41, 2000 (PubMed).