产品详情
  • 产品名称:SemaDomain,ImmunoglobulinDomain(Ig),TransmembraneDomain(TM)andShortCytoplasmicD

  • 产品型号:
  • 产品厂商:ACROBiosystems
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简单介绍:
SemaDomain,ImmunoglobulinDomain(Ig),TransmembraneDomain(TM)andShortCytoplasmicDomain,(Semaphorin)4D(SEMA4D)(AA22-734)(Active)protein(FcTag)
详情介绍:
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 105.4 kDa. The protein migrates as 120-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to promote the survival and differentiation of human peripheral blood monocytes. The ED50 for this effect is typically 2.0-6.3 μg/mL.

SDS-PAGE: due to glycosylation.
Background Cluster of Differentiation 100 (CD100) is also known as Semaphorin-4D (SEMA4D), is a single-pass type I membrane protein which belongs to the semaphorin family, and is a human 150- kDa homodimer expressed at the surface of most hemopoietic cells. Semaphorin genes encode soluble and membrane-bound proteins, most of which have been shown to act as chemorepellents on growth cone guidance. CD100 is discrete, as it is a transmembrane leukocyte surface molecule that can also exist in a soluble form. Semaphorin 4D (Sema 4D) is an axon guidance molecule which is secreted by oligodendrocytes and induces growth cone collapse in the central nervous system. By binding plexin B1 receptor it functions as an R-Ras GTPase-activating protein (GAP) and repels axon growth cones in both the mature central nervous system. In the immune system, CD100 binds CD72 to activate B cells and dendritic cells, though much about this interaction is still under investigation. It is involved in oligodendrogenesis during development and during recovery from ischemic injury.
Molecular Weight 105.4 kDa
UniProt Q92854
Research Area Neurology, CD Antigens
Pathways Regulation of Cell Size
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (AA 22-734) (Active) protein (Fc Tag) (ABIN2181739) Human Semaphorin 4D, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was sta...
Background publications Delaire, Billard, Tordjman, Chédotal, Elhabazi, Bensussan, Boumsell: "Biological activity of soluble CD100. II. Soluble CD100, similarly to H-SemaIII, inhibits immune cell migration." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 7, pp. 4348-54, 2001 (PubMed).