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  • 产品名称：TumorNecrosisFactorReceptorSuperfamily,Member10b(TNFRSF10B)(AA56-182)(Active)pr

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  • 产品厂商：ACROBiosystems
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简单介绍：
TumorNecrosisFactorReceptorSuperfamily,Member10b(TNFRSF10B)(AA56-182)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.1 kDa. The protein migrates as 15-20 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized human TNFRSF10B at 10 μg/mL ( 100 μL/well ) can bind biotinylated TNFSF10 with a linear range of 0.375 - 10 ng/mL.
Background Tumor necrosis factor receptor superfamily member 10B (TNFRSF10B) is also known as TNF-related apoptosis-inducing ligand receptor 2 (TRAILR2), Death receptor 5 (DR5), CD262, KILLER, is a member of the TNF-receptor superfamily, and contains an intracellular death domain. TNFRSF10B / DR-5 is widely expressed in ***** and fetal tissues, very highly expressed in tumor cell lines. TRAILR2 / CD262 / DR5 is the receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD (a death domain containing adaptor protein) of TRAIL-R2 / TNFRSF10B recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. CD262 / DR5 Promotes the activation of NF-kappa-B. DR5 is essential for ER stress-induced apoptosis and is regulated by p53/TP53.
Molecular Weight 15.1 kDa
NCBI Accession NP_003833
UniProt O14763
Pathways p53 Signaling, Apoptosis, Positive Regulation of Endopeptidase Activity
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B) (AA 56-182) (Active) protein (His tag) (ABIN2181866) Human TRAIL R2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained...
Background publications Yamaguchi, Wang: "CHOP is involved in endoplasmic reticulum stress-induced apoptosis by enhancing DR5 expression in human carcinoma cells." in: The Journal of biological chemistry, Vol. 279, Issue 44, pp. 45495-502, 2004 (PubMed).