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  • 产品名称：CD14Molecule(CD14)(AA20-352)(Active)protein(FcTag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CD14Molecule(CD14)(AA20-352)(Active)protein(FcTag)
详情介绍：
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 62.4 kDa. The protein migrates as 75-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >97 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to enhance LPS-stimulated IL-8 secretion by THP-1 human acute monocytic leukemia cells. The ED50 for this effect is typically 0.5-4 μg/mL.
Background Cluster of differentiation 14 (CD14), is a cell surface glycoprotein, and is a is a component of the innate immune system. CD14 is a myelomonocytic differentiation antigen preferentially expressed on monocytes, macrophages, and activated granulocytes. CD14 exists in two forms. Either it is anchored into the membrane by a glycosylphosphatidylinositol tail (mCD14) or it appears in a soluble form (sCD14). Soluble CD14 either appears after shedding of mCD14 (48 kDa) or is directly secreted from intracellular vesicles (56 kDa). CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). CD14 has been proposed to be involved in various biological processes, including transportation of other lipids, cell-cell interaction during different immune responses, as well as recognition of apoptotic cells. Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns. CD14+ cells are monocytes that can differentiate into a host of different cells. CD14 has been shown to interact with Lipopolysaccharide-binding protein.
Molecular Weight 62.4 kDa
UniProt P08571
Research Area Stem Cells, Innate Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Immunology, Inflammation
Pathways TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for CD14 Molecule (CD14) (AA 20-352) (Active) protein (Fc Tag) (ABIN2180688) Human CD14, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained over...
Background publications Thomas, Kapoor, Sharma, Bausinger, Zyilan, Lipsker, Hanau, Surolia: "Evidence of a trimolecular complex involving LPS, LPS binding protein and soluble CD14 as an effector of LPS response." in: FEBS letters, Vol. 531, Issue 2, pp. 184-8, 2002 (PubMed).

Kitchens: "Role of CD14 in cellular recognition of bacterial lipopolysaccharides." in: Chemical immunology, Vol. 74, pp. 61-82, 2000 (PubMed).

Tapping, Tobias: "Soluble CD14-mediated cellular responses to lipopolysaccharide." in: Chemical immunology, Vol. 74, pp. 108-21, 2000 (PubMed).

Kirkland, Viriyakosol: "Structure-function analysis of soluble and membrane-bound CD14." in: Progress in clinical and biological research, Vol. 397, pp. 79-87, 1998 (PubMed).