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  • 产品名称：V-Erb-AerythroblasticLeukemiaViralOncogeneHomolog4(Avian)(ERBB4)(AA26-651)(Acti

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  • 产品厂商：ACROBiosystems
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简单介绍：
V-Erb-AerythroblasticLeukemiaViralOncogeneHomolog4(Avian)(ERBB4)(AA26-651)(Active)protein(FcTag)
详情介绍：
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 96 kDa. The protein migrates as 122-132 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >90 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to inhibit the biological activity of Neuregulin-1-β1 on MCF-7 human breast cancer cells. In the presence of 10 ng/mL of Recombinant Human NRG1-β1/HRG1-β1 Extracellular Domain. The ED50 for this effect is typically 1.0-5.5 μg/mL.
Background Receptor tyrosine-protein kinase erbB-4 (ErbB4) is also known as Her4, is a single-pass type I transmembrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB4 is expressed in normal skeletal muscle, heart, pituitary, brain and several breast carcinomas. ERBB4 contains multiple furin-like cysteine rich domains, a tyrosine kinase domain, a phosphotidylinositol-3 kinase and a PDZ domain binding motif. The protein binds to and is activated by neuregulins-2 and -3, heparin-binding EGF-like growth factor and betacellulin. Ligand binding induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. ErbB4 appears to play important roles in neuronal development, development of the heart and cancer. ERBB4 has been shown to interact with: DLG4, NRG1, STAT5A and YAP1. Mutations in this gene have been associated with cancer. Other single-nucleotide polymorphisms and a risk haplotype have been linked to schizophrenia.
Molecular Weight 96 kDa
NCBI Accession NP_001036064
UniProt Q15303
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) (AA 26-651) (Active) protein (Fc Tag) (ABIN2181046) Human ErbB4, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained ove...
Background publications Silberberg, Darvasi, Pinkas-Kramarski, Navon: "The involvement of ErbB4 with schizophrenia: association and expression studies." in: American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics, Vol. 141B, Issue 2, pp. 142-8, 2006 (PubMed).

Garcia, Vasudevan, Buonanno: "The neuregulin receptor ErbB-4 interacts with PDZ-containing proteins at neuronal synapses." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 7, pp. 3596-601, 2000 (PubMed).