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  • 产品名称：EpithelialCellAdhesionMolecule(EPCAM)(AA24-265)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
EpithelialCellAdhesionMolecule(EPCAM)(AA24-265)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 29 kDa. The protein migrates as 33-36 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant human Cathepsin V at 10 μg/mL ( 100 μL/well ) can bind biotinylated EpCAM. The EC50 of biotinylated EpCAM is 50-70 ng/mL

SDS-PAGE: rhEpCAM protein migrates as 33-36 kDa poly peptide due to glycosylation.
Background EpCAM is also known as CO171A, EGP, EGP40, GA7332, KSA, M4S, MIC18, MK1, TROP1, hEGP2, and is a pan-epithelial differentiation antigen that is expressed on almost all carcinomas as 17-1A(mAb) antigen. Its constitutional function is being elucidated. It is intricately linked with the Cadherin-Catenin pathway and hence the fundamental WNT pathway responsible for intracellular signaling and polarity. The epithelial cell adhesion molecule (Ep-CAM) is known to express in most epithelial malignancies and was reported as a tumor marker or a candidate of molecular targeting therapy. Ep-CAM cross signaling with N-cadherin involves Pi3K, resulting in the abrogation of the cadherin adhesion complexes in epithelial cells was reported. And Epithelial cell adhesion molecule (Ep-CAM) recently received increased attention as a prognostic factor in breast cancer.
Molecular Weight 29 kDa
UniProt P16422
Research Area Cancer
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Epithelial Cell Adhesion Molecule (EPCAM) (AA 24-265) (Active) protein (His tag) (ABIN2181014) Human EpCAM, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ov...
Product cited in: Zielonka, Weber, Becker, Doerner, Christmann, Christmann, Uth, Fritz, Schäfer, Steinmann, Empting, Ockelmann, Lierz, Kolmar: "Shark Attack: high affinity binding proteins derived from shark vNAR domains by stepwise in vitro affinity maturation." in: Journal of biotechnology, Vol. 191, pp. 236-45, 2014 (PubMed).