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  • 产品名称：CellAdhesionMolecule1(CADM1)(AA45-374)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CellAdhesionMolecule1(CADM1)(AA45-374)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 38.5 kDa. The protein migrates as 38.5 kDa, 50 kDa and 55-85 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized rhCADM1 at 2 μg/mL ( 100 μL/well ) can bind biotinylated human CRTAM with a linear range of 12.5 - 500 ng/mL.

SDS-PAGE: rhCADM1 protein migrates as 38.5kDa, 50kDa and 55-85 kDa poly peptide due to different glycosylation.
Background Cell Adhesion Molecules (CAMs) are proteins located on the cell surface involved with the binding with other cells or with the extracellular matrix (ECM) in the process called cell adhesion. Cell adhesion molecule 1 is a single-pass type I membrane protein which belongs to the nectin family. Cell Adhesion Molecule 1 contains two Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain, and is also known as CADM1, BL2, DKFZp686F1789, IGSF4, IGSF4A, MGC149785, MGC51880, NECL2, Necl-2, RA175, ST17, SYNCAM, TSLC1, sTSLC-1, sgIGSF, synCAM1. CADM1 mediates heterophilic cell-cell adhesion with CADM3 and PVRL3 in a Ca2+-independent manner. CADM1 acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. CADM1 may be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. CADM1 may play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa.
Molecular Weight 38.5 kDa
NCBI Accession NP_055148
UniProt Q9BY67
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Activated T Cell Proliferation
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Cell Adhesion Molecule 1 (CADM1) (AA 45-374) (Active) protein (His tag) (ABIN2180672) Human CADM1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ov...
Background publications Dong, Xu, Gong, Gao, Lin, Chen, Peng, Qiang, Yuan, Peng, Rao: "Crystal structure of the V domain of human Nectin-like molecule-1/Syncam3/Tsll1/Igsf4b, a neural tissue-specific immunoglobulin-like cell-cell adhesion molecule." in: The Journal of biological chemistry, Vol. 281, Issue 15, pp. 10610-7, 2006 (PubMed).

Boles, Barchet, Diacovo, Cella, Colonna: "The tumor suppressor TSLC1/NECL-2 triggers NK-cell and CD8+ T-cell responses through the cell-surface receptor CRTAM." in: Blood, Vol. 106, Issue 3, pp. 779-86, 2005 (PubMed).

Furuno, Ito, Koma, Watabe, Yokozaki, Bienenstock, Nakanishi, Kitamura: "The spermatogenic Ig superfamily/synaptic cell adhesion molecule mast-cell adhesion molecule promotes interaction with nerves." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 11, pp. 6934-42, 2005 (PubMed).

Yageta, Kuramochi, Masuda, Fukami, Fukuhara, Maruyama, Shibuya, Murakami: "Direct association of TSLC1 and DAL-1, two distinct tumor suppressor proteins in lung cancer." in: Cancer research, Vol. 62, Issue 18, pp. 5129-33, 2002 (PubMed).

Kuramochi, Fukuhara, Nobukuni, Kanbe, Maruyama, Ghosh, Pletcher, Isomura, Onizuka, Kitamura, Sekiya, Reeves, Murakami: "TSLC1 is a tumor-suppressor gene in human non-small-cell lung cancer." in: Nature genetics, Vol. 27, Issue 4, pp. 427-30, 2001 (PubMed).