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  • 产品名称：ActivatedLeukocyteCellAdhesionMolecule(ALCAM)(AA28-526)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
ActivatedLeukocyteCellAdhesionMolecule(ALCAM)(AA28-526)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 56.7 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized rh CD166 / ALCAM at 10 μg/mL (100 μL/well) can bind mouse CD6 Fc Chimera, The EC50 of mouse CD6 Fc Chimera is 0.1-0.22 μg/mL.

SDS-PAGE: due to glycosylation.
Background CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins, which is also known as Activated leukocyte cell adhesion molecule (ALCAM) in human, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. CD166 is a cell adhesion molecule that binds to CD6. CD166 involved in neurite extension by neurons via heterophilic and homophilic interactions.
Molecular Weight 56.7 kDa
NCBI Accession NP_001618
UniProt Q13740
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Activated Leukocyte Cell Adhesion Molecule (ALCAM) (AA 28-526) (Active) protein (His tag) (ABIN2180701) Human ALCAM, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ov...
Background publications Skonier, Bowen, Emswiler, Aruffo, Bajorath: "Recognition of diverse proteins by members of the immunoglobulin superfamily: delineation of the receptor in the human CD6 ligand ALCAM." in: Biochemistry, Vol. 35, Issue 38, pp. 12287-91, 1996 (PubMed).

Bajorath, Bowen, Aruffo: "Molecular model of the N-terminal receptor-binding domain of the human CD6 ligand ALCAM." in: Protein science : a publication of the Protein Society, Vol. 4, Issue 8, pp. 1644-7, 1996 (PubMed).