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  • 产品名称：NeurotrophicTyrosineKinase,Receptor,Type2(NTRK2)(AA32-430)(Active)protein(FcTag

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  • 产品厂商：ACROBiosystems
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简单介绍：
NeurotrophicTyrosineKinase,Receptor,Type2(NTRK2)(AA32-430)(Active)protein(FcTag)
详情介绍：
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 70.4 kDa. The protein migrates as 95-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >98 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to bind mouse BDNF in functional ELISA.
Background Neurotrophic tyrosine kinase receptor type 2 (NTRK2) is also known as BDNF/NT-3 growth factors receptor, Tropomyosin-related kinase B (TRKB) and TrkB tyrosine kinase, which belongs to the protein kinase superfamily or Tyr protein kinase family. Insulin receptor subfamily. NTRK2 / TrkB contains two Ig-like C2-type (immunoglobulin-like) domains, two LRR (leucine-rich) repeats, one LRRCT domain, one LRRNT domain, one protein kinase domain. NTRK2 / Trk-B is expressed in the central and peripheral nervous system. The catalytic activity of NTRK2 is ""ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate"". NTRK2 / TrkB involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity.
Molecular Weight 70.4 kDa
UniProt Q16620
Research Area Cancer
Pathways RTK Signaling, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Skeletal Muscle Fiber Development, Feeding Behaviour, Dicarboxylic Acid Transport
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) (AA 32-430) (Active) protein (Fc Tag) (ABIN2181553) Human TrkB, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained over...
Background publications Yeo, Connie Hung, Rochford, Keogh, Gray, Sivaramakrishnan, ORahilly, Farooqi: "A de novo mutation affecting human TrkB associated with severe obesity and developmental delay." in: Nature neuroscience, Vol. 7, Issue 11, pp. 1187-9, 2004 (PubMed).

Stoilov, Castren, Stamm: "Analysis of the human TrkB gene genomic organization reveals novel TrkB isoforms, unusual gene length, and splicing mechanism." in: Biochemical and biophysical research communications, Vol. 290, Issue 3, pp. 1054-65, 2002 (PubMed).

Meakin, MacDonald, Gryz, Kubu, Verdi: "The signaling adapter FRS-2 competes with Shc for binding to the nerve growth factor receptor TrkA. A model for discriminating proliferation and differentiation." in: The Journal of biological chemistry, Vol. 274, Issue 14, pp. 9861-70, 1999 (PubMed).

Allen, Dawbarn, Eckford, Wilcock, Ashcroft, Colebrook, Feeney, MacGowan: "Cloning of a non-catalytic form of human trkB and distribution of messenger RNA for trkB in human brain." in: Neuroscience, Vol. 60, Issue 3, pp. 825-34, 1994 (PubMed).