产品详情
查看大图

  • 产品名称：IsocitrateDehydrogenase1(NADP+),Soluble(IDH1)(AA1-414)(Active)protein(Histag)

  • 产品型号：
  • 产品厂商：ACROBiosystems
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
IsocitrateDehydrogenase1(NADP+),Soluble(IDH1)(AA1-414)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 47.5 kDa. The protein migrates as 47.5 kDa under reducing (R) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Isocitrate dehydrogenase 1 (IDH1) is also known as Cytosolic NADP-isocitrate dehydrogenase (NADP(+)-specific ICDH), Oxalosuccinate decarboxylase and PICD, which is one of the Isocitrate dehydrogenases that can catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD+ as the electron acceptor and the other NADP+. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.
Molecular Weight 47.5 kDa
NCBI Accession NP_005887
UniProt O75874
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 150 mM NaCl, 0.05 % Brij-35, 1 mM DTT, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 1-414) (Active) protein (His tag) (ABIN2181246) Human IDH1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ove...
Background publications Dang, White, Gross, Bennett, Bittinger, Driggers, Fantin, Jang, Jin, Keenan, Marks, Prins, Ward, Yen, Liau, Rabinowitz, Cantley, Thompson, Vander Heiden, Su: "Cancer-associated IDH1 mutations produce 2-hydroxyglutarate." in: Nature, Vol. 462, Issue 7274, pp. 739-44, 2009 (PubMed).

Choudhary, Kumar, Gnad, Nielsen, Rehman, Walther, Olsen, Mann: "Lysine acetylation targets protein complexes and co-regulates major cellular functions." in: Science (New York, N.Y.), Vol. 325, Issue 5942, pp. 834-40, 2009 (PubMed).

Choudhary, Olsen, Brandts, Cox, Reddy, Böhmer, Gerke, Schmidt-Arras, Berdel, Müller-Tidow, Mann, Serve: "Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes." in: Molecular cell, Vol. 36, Issue 2, pp. 326-39, 2009 (PubMed).

Xu, Zhao, Xu, Peng, Huang, Arnold, Ding: "Structures of human cytosolic NADP-dependent isocitrate dehydrogenase reveal a novel self-regulatory mechanism of activity." in: The Journal of biological chemistry, Vol. 279, Issue 32, pp. 33946-57, 2004 (PubMed).