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  • 产品名称：Mesothelin(MSLN)(AA296-580)(Active)protein(FcTag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
Mesothelin(MSLN)(AA296-580)(Active)protein(FcTag)
详情介绍：
Characteristics This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 59.6 kDa. As a result of glycosylation, the protein migrates as 60-68 kDa under reducing (R) condition, and 120-130 kDa under non-reducing (NR) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.
Molecular Weight 59.6 kDa
UniProt Q13421
Research Area Cancer
Pathways EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Mesothelin (MSLN) (AA 296-580) (Active) protein (Fc Tag) (ABIN2181518) Human Mesothelin (aa 296-580), Fc Tag on SDS-PAGE under reducing (R) and no-reducing ...
Background publications Ma, Tang, Esser, Pastan, Xia: "Recognition of mesothelin by the therapeutic antibody MORAb-009: structural and mechanistic insights." in: The Journal of biological chemistry, Vol. 287, Issue 40, pp. 33123-31, 2012 (PubMed).

Rump, Morikawa, Tanaka, Minami, Umesaki, Takeuchi, Miyajima: "Binding of ovarian cancer antigen CA125/MUC16 to mesothelin mediates cell adhesion." in: The Journal of biological chemistry, Vol. 279, Issue 10, pp. 9190-8, 2004 (PubMed).

Chang, Pastan: "Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 1, pp. 136-40, 1996 (PubMed).