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  • 产品名称：FcFragmentofIgE,HighAffinityI,ReceptorFor,alphaPolypeptide(FCER1A)(AA26-205)(Ac

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  • 产品厂商：ACROBiosystems
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简单介绍：
FcFragmentofIgE,HighAffinityI,ReceptorFor,alphaPolypeptide(FCER1A)(AA26-205)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 21.9 kDa. The protein migrates as 40-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >90 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized human FCER1A at 2 μg/mL (100 μL/well). The concentration of Human IgE that produces 50% of the optimal binding response is found to be approximately 0.1 - 0.4 μg/mL.
Background High affinity immunoglobulin epsilon receptor subunit alpha (FCER1A) is also known as Fc-epsilon RI-alpha (FcERI), IgE Fc receptor subunit alpha, FCE1A. FCER1A contains two Ig-like (immunoglobulin-like) domains. FCER1A binds to the Fc region of immunoglobulins epsilon and is a high affinity receptor. FCER1A is responsible for initiating the allergic response, which binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines. FCER1A plays a central role in allergic disease, coupling allergen and mast cell to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma.
Molecular Weight 21.9 kDa
NCBI Accession NP_001992
UniProt P12319
Pathways Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Fc Fragment of IgE, High Affinity I, Receptor For, alpha Polypeptide (FCER1A) (AA 26-205) (Active) protein (His tag) (ABIN2181074) Human Fc epsilon RI alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel ...
Background publications Garman, Sechi, Kinet, Jardetzky: "The analysis of the human high affinity IgE receptor Fc epsilon Ri alpha from multiple crystal forms." in: Journal of molecular biology, Vol. 311, Issue 5, pp. 1049-62, 2001 (PubMed).

Garman, Kinet, Jardetzky: "Crystal structure of the human high-affinity IgE receptor." in: Cell, Vol. 95, Issue 7, pp. 951-61, 1999 (PubMed).

Yagi, Yanagida, Tanida, Hasegawa, Okumura, Ra et al.: "High-level expression of the truncated alpha chain of human high-affinity receptor for IgE as a soluble form by baculovirus-infected insect cells. Biochemical characterization of the recombinant ..." in: European journal of biochemistry / FEBS, Vol. 220, Issue 2, pp. 593-8, 1994 (PubMed).