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  • 产品名称：S100CalciumBindingProteinB(S100B)(AA1-92)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
S100CalciumBindingProteinB(S100B)(AA1-92)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 11.5 kDa. The protein migrates as 11 kDa under reducing (R) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to bind human AGER in a functional ELISA.
Background S-100 protein beta chain (S100B),a member of the S-100 family, is also known as S-100 protein subunit beta and S100 calcium-binding protein B, which contains two EF-hand domains. S100B can weakly bind calcium but bind zinc very tightly-distinct s with different affinities exist for both ions on each monomer. Also, S100B can bind to and initiate the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction,S100B may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Fuethermore,S100B could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. S100B may mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.
Molecular Weight 11.5 kDa
NCBI Accession NP_006263
UniProt P04271
Pathways Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Regulation of long-term Neuronal Synaptic Plasticity
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 150 mM NaCl, pH 8.0
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for S100 Calcium Binding Protein B (S100B) (AA 1-92) (Active) protein (His tag) (ABIN2181721) Human S100B, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ov...
Background publications Yamaguchi, Umeda, Shimamoto, Tsuchiya, Tokumitsu, Tokuda, Kobayashi: "S100 proteins modulate protein phosphatase 5 function: a link between CA2+ signal transduction and protein dephosphorylation." in: The Journal of biological chemistry, Vol. 287, Issue 17, pp. 13787-98, 2012 (PubMed).

McClintock, Shaw: "A novel S100 target conformation is revealed by the solution structure of the Ca2+-S100B-TRTK-12 complex." in: The Journal of biological chemistry, Vol. 278, Issue 8, pp. 6251-7, 2003 (PubMed).

Smith, Shaw: "A novel calcium-sensitive switch revealed by the structure of human S100B in the calcium-bound form." in: Structure (London, England : 1993), Vol. 6, Issue 2, pp. 211-22, 1998 (PubMed).