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  • 产品名称：ParathyroidHormone1Receptor(PTH1R)(AA27-188)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
ParathyroidHormone1Receptor(PTH1R)(AA27-188)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 19.3 kDa. The protein migrates as 23-40 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. When rhPTH1R is used at 2 μg/mL, the concentration of biotinylated human PTHrP that produces 50% of the optimal binding response is found to be approximately 0.1 - 0.25 μg/mL.
Background Parathyroid hormone/parathyroid hormone-related peptide receptor is also known as Parathyroid hormone 1 receptor (PTH1R), PTHR1, PTH/PTHrP type I receptor (PTH/PTHr receptor), which belongs to the G-protein coupled receptor 2 family. PTH1R / PTHR1 is a receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.
Molecular Weight 19.3 kDa
NCBI Accession NP_000307
UniProt Q03431
Pathways Regulation of Carbohydrate Metabolic Process
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Parathyroid Hormone 1 Receptor (PTH1R) (AA 27-188) (Active) protein (His tag) (ABIN2181675) Human PTH1R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ov...
Background publications Pal, Swaminathan, Xu, Pioszak: "Structural basis for hormone recognition by the Human CRFR2{alpha} G protein-coupled receptor." in: The Journal of biological chemistry, Vol. 285, Issue 51, pp. 40351-61, 2010 (PubMed).

Pioszak, Harikumar, Parker, Miller, Xu: "Dimeric arrangement of the parathyroid hormone receptor and a structural mechanism for ligand-induced dissociation." in: The Journal of biological chemistry, Vol. 285, Issue 16, pp. 12435-44, 2010 (PubMed).

Johnston, Kimple, Giguère, Siderovski: "Structure of the parathyroid hormone receptor C terminus bound to the G-protein dimer Gbeta1gamma2." in: Structure (London, England : 1993), Vol. 16, Issue 7, pp. 1086-94, 2008 (PubMed).