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  • 产品名称：delta-Like4(DLL4)(AA27-524)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
delta-Like4(DLL4)(AA27-524)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 55.1 kDa. The protein migrates as 65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >92 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to bind human Notch1 in a functional ELISA.

SDS-PAGE: due to glycosylation.
Background Delta-like protein 4 (DLL4) is also known as Drosophila Delta homolog 4 (Delta4), which contains one DSL domain and eight EGF-like domains. DLL4 is expressed in vascular endothelium. DLL4 is involved in the Notch signaling pathway as Notch ligand, which can activates NOTCH1 and NOTCH4. DLL4 is involved in angiogenesis and negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. DLL4 can bind to Notch-1 and Notch-4.
Molecular Weight 55.1 kDa
UniProt Q9NR61
Research Area Chromatin and Nuclear Signaling, Cell Cycle
Pathways Notch Signaling
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for delta-Like 4 (DLL4) (AA 27-524) (Active) protein (His tag) (ABIN2180971) Human DLL4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ove...
Background publications Brütsch, Liebler, Wüstehube, Bartol, Herberich, Adam, Telzerow, Augustin, Fischer: "Integrin cytoplasmic domain-associated protein-1 attenuates sprouting angiogenesis." in: Circulation research, Vol. 107, Issue 5, pp. 592-601, 2010 (PubMed).

Corazza, Rennella, Schanda, Mimmi, Cutuil, Raimondi, Giorgetti, Fogolari, Viglino, Frydman, Gal, Bellotti, Brutscher, Esposito: "Native-unlike long-lived intermediates along the folding pathway of the amyloidogenic protein beta2-microglobulin revealed by real-time two-dimensional NMR." in: The Journal of biological chemistry, Vol. 285, Issue 8, pp. 5827-35, 2010 (PubMed).

Del Barrio, Taveira-Marques, Muroyama, Yuk, Li, Wines-Samuelson, Shen, Smith, Xiang, Rowitch, Richardson: "A regulatory network involving Foxn4, Mash1 and delta-like 4/Notch1 generates V2a and V2b spinal interneurons from a common progenitor pool." in: Development (Cambridge, England), Vol. 134, Issue 19, pp. 3427-36, 2007 (PubMed).