产品详情
查看大图

  • 产品名称：CD48Molecule(CD48)(AA27-220)(Active)protein(Histag)

  • 产品型号：
  • 产品厂商：ACROBiosystems
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
CD48Molecule(CD48)(AA27-220)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 23.2 kDa. The protein migrates as 38-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD244 protein Fc Chimera at 5 μg/mL (100μL/well) can bind human CD48 with a linear range of 0.1- 4 μg/mL.
Background CD48 antigen is also known as Signaling lymphocytic activation molecule 2, SLAM family member 2 (SLAMF2), Leukocyte antigen MEM-102, BCM1 surface antigen, B-lymphocyte activation marker BLAST-1. CD48 is a 65 kDa GPI-linked protein which belongs to the CD2 subfamily of immunoglobulin superfamily molecules. CD48 antigen contains two Ig-like C2-type (immunoglobulin-like) domains and two Ig-like V-type (immunoglobulin-like) domain. CD48 interacts with CD244 in an heterophilic manner. CD48 is ligand for CD2. CD48 might facilitate interaction between activated lymphocytes. CD48 is probably involved in regulating T-cell activation.
Molecular Weight 23.2 kDa
UniProt P09326
Research Area CD Antigens, Surface Receptors of Immune Cells
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for CD48 Molecule (CD48) (AA 27-220) (Active) protein (His tag) (ABIN2180807) Human CD48, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ove...
Background publications Wollscheid, Bausch-Fluck, Henderson, OBrien, Bibel, Schiess, Aebersold, Watts: "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." in: Nature biotechnology, Vol. 27, Issue 4, pp. 378-86, 2009 (PubMed).

Korínek, Stefanová, Angelisová, Hilgert, Horejsí: "The human leucocyte antigen CD48 (MEM-102) is closely related to the activation marker Blast-1." in: Immunogenetics, Vol. 33, Issue 2, pp. 108-12, 1991 (PubMed).