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  • 产品名称：EphrinA1(EFNA1)(AA19-182)(Active)protein(FcTag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
EphrinA1(EFNA1)(AA19-182)(Active)protein(FcTag)
详情介绍：
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 46 kDa. As a result of glycosylation, the protein migrates as 50-55 kDa under reducing (R) condition, and 100-110 kDa under non-reducing (NR) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. When Recombinant Human EphA2 is coated at 4 μg/mL (100 μL/well), the concentration of rhEphrin-A1 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 0.5 - 4 ng/mL.
Background Ephrin-A1 (EFNA1) is also known as EPLG1, LERK1, TNFAIP4, Immediate early response protein B61, which belongs to the ephrin family. EFNA1 contains one ephrin RBD (ephrin receptor-binding) domain. EFNA1 is cell surface GPI-bound ligand for Eph receptors, and is a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. EFNA1 binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. EFNA1 plays an important role in angiogenesis and tumor neovascularization.
Molecular Weight 46 kDa
NCBI Accession NP_004419
UniProt P20827
Pathways RTK Signaling
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Ephrin A1 (EFNA1) (AA 19-182) (Active) protein (Fc Tag) (ABIN2180992) Human Ephrin-A1, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) condition...
Background publications Wykosky, Palma, Gibo, Ringler, Turner, Debinski: "Soluble monomeric EphrinA1 is released from tumor cells and is a functional ligand for the EphA2 receptor." in: Oncogene, Vol. 27, Issue 58, pp. 7260-73, 2008 (PubMed).

Wykosky, Gibo, Stanton, Debinski: "Interleukin-13 receptor alpha 2, EphA2, and Fos-related antigen 1 as molecular denominators of high-grade astrocytomas and specific targets for combinatorial therapy." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 1, pp. 199-208, 2008 (PubMed).

Liu, Wang, Koeffler, Xie: "Ephrin-A1 is a negative regulator in glioma through down-regulation of EphA2 and FAK." in: International journal of oncology, Vol. 30, Issue 4, pp. 865-71, 2007 (PubMed).