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  • 产品名称：tyrosine3-Monooxygenase/tryptophan5-MonooxygenaseActivationProtein,betaPolypept

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  • 产品厂商：ACROBiosystems
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简单介绍：
tyrosine3-Monooxygenase/tryptophan5-MonooxygenaseActivationProtein,betaPolypeptide(YWHAB)(AA1-246)(Active)protein(GSTtag)
详情介绍：
Characteristics This protein carries a GST tag at the N-terminus. The protein has a calculated MW of 55 kDa. The protein migrates as 53 kDa under reducing (R) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide (YWHAB) is also known as 14-3-3 protein beta, GW128, HS1. YWHAB belongs to the 14-3-3 family. YWHAB binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. YWHAB is negative regulator of osteogenesis. YWHAB blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2.
Molecular Weight 55 kDa
NCBI Accession NP_647539
UniProt P31946
Pathways Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, Maintenance of Protein Location
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 150 mM NaCl, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, beta Polypeptide (YWHAB) (AA 1-246) (Active) protein (GST tag) (ABIN2181927) Human YWHAB, GST Tag on SDS-PAGE under reducing (R) condition. The gel was stained ov...
Background publications Jang, Liu, Fu, Rees, Yepes, Levey, Ye: "Interaction of Akt-phosphorylated SRPK2 with 14-3-3 mediates cell cycle and cell death in neurons." in: The Journal of biological chemistry, Vol. 284, Issue 36, pp. 24512-25, 2009 (PubMed).

Liu, Ross, Bodine, Billiard: "Homodimerization of Ror2 tyrosine kinase receptor induces 14-3-3(beta) phosphorylation and promotes osteoblast differentiation and bone formation." in: Molecular endocrinology (Baltimore, Md.), Vol. 21, Issue 12, pp. 3050-61, 2007 (PubMed).