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  • 产品名称：EpidermalGrowthFactorReceptor(EGFR)(AA25-645)(Active)protein(FcTag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
EpidermalGrowthFactorReceptor(EGFR)(AA25-645)(Active)protein(FcTag)
详情介绍：
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 95 kDa. The protein migrates as 130-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant human EGF (Catalog # EGF-H4114) at 10 μg /mL (100 μL/well ) can bind rhEGFR Fc Chimera (Catalog # EGR-H5252) with a linear range of 0.025-0.5 μg /mL. Measured by its ability to bind human EGF (Cat# EGF-H4114) in the SPR assay (Biacore 2000) with an estimated KD < 1nM. Validated CetuxiMab binding in a functional ELISA.

SDS-PAGE: due to glycosylation.
Background The epidermal growth factor receptor (EGFR, ErbB-1, HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.
Molecular Weight 95 kDa
NCBI Accession NP_005219
UniProt P00533
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Epidermal Growth Factor Receptor (EGFR) (AA 25-645) (Active) protein (Fc Tag) (ABIN2181000) Human EGF R, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained ove...
Product cited in: Singer, Fazekas, Wang, Weichselbaumer, Matz, Mader, Steinfellner, Meitz, Mechtcheriakova, Sobanov, Willmann, Stockner, Spillner, Kunert, Jensen-Jarolim: "Generation of a canine anti-EGFR (ErbB-1) antibody for passive immunotherapy in dog cancer patients." in: Molecular cancer therapeutics, Vol. 13, Issue 7, pp. 1777-90, 2014 (PubMed).