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产品名称:CytotoxicT-Lymphocyte-AssociatedProtein4(CTLA4)(AA37-160)(Active)protein(FcTag)
产品型号:
产品厂商:ACROBiosystems
产品文档:
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简单介绍:
CytotoxicT-Lymphocyte-AssociatedProtein4(CTLA4)(AA37-160)(Active)protein(FcTag)
详情介绍:
Product details
Product details
Characteristics
This protein carries a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 40 kDa. The predicted N-terminus is Ala 37. As a result of glycosylation, the reducing (R) protein migrates as 50-55 kDa, and the non-reducing (NR) protein migrates as 100-110 kDa in SDS-PAGE.
Purity
>98 % as determined by SDS-PAGE.
Sterility
0.22 μm filtered
Endotoxin Level
Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment
Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CTLA4 Protein Fc Chimera (Catalog # CT4-H5255) at 5 μg/mL (100 μL/well) can bind rhB7-1/ CD80 Protein (Catalog # B71-H5228) with a linear range of 0.1 - 2 μg/mL. Measured by its binding ability in a functional ELISA. Immobilized Human CTLA4 Protein Fc Chimera (Catalog # CT4-H5255) at 5 μg/mL (100 μL/well) can bind rh PD-L1 /B7-H1 /CD274 Protein (Catalog # PD1-H5229) with a linear range of 1 - 10 ug/mL.
Target details
Target details
Background
CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.
Molecular Weight
40 kDa
NCBI Accession
NP_005205
UniProt
P16410
Application Details
Application Details
Restrictions
For Research Use only
Handling
Handling
Format
Lyophilized
Reconstitution
Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer
35 mM Tris, 50 mM NaCl, 50 mM Glycine, pH 8.0
Handling Advice
Avoid repeated freeze-thaw cycles.
Storage
-20 °C
Storage Comment
No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Images
Images
Supplier Images
Human CTLA-4, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. ...
Immobilized Human CTLA-4, Fc Tag (Cat# CT4-H5255) at 10 μg/mL (100 µl/well), can bind...
Immobilized Human B7-2, His Tag (Cat# CD6-H5223) at 10 μg/mL (100 µl/well), can bind ...
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Images for product: Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 37-160) (Active) protein (Fc Tag)
Human CTLA-4, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.
Immobilized Human CTLA-4, Fc Tag (Cat# CT4-H5255) at 10 μg/mL (100 µl/well), can bind Human B7-1, His Tag (Cat# B71-H5228) with a linear range of 0.04-4 μg/mL.
Immobilized Human B7-2, His Tag (Cat# CD6-H5223) at 10 μg/mL (100 µl/well), can bind Human CTLA-4, Fc Tag (Cat# CT4-H5255) with a linear range of 0.04-0.3 μg/mL.
References
References
Background publications
Magistrelli, Jeannin, Herbault, Benoit De Coignac, Gauchat, Bonnefoy, Delneste: "A soluble form of CTLA-4 generated by alternative splicing is expressed by nonstimulated human T cells."
in:
European journal of immunology
, Vol. 29, Issue 11, pp. 3596-602, 1999 (PubMed).