产品详情
查看大图

  • 产品名称：CathepsinL1(CTSL1)(AA18-333)(Active)protein(Histag)

  • 产品型号：
  • 产品厂商：ACROBiosystems
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
CathepsinL1(CTSL1)(AA18-333)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 36.7 kDa. The protein migrates as 10 kDa and 34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation,corresponding to the propeptide and the mature form respectively..
Purity >98 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD74 at 5 μg/mL ( 100 μL/well ) can bind biotinylated human CTSL1 with a linear range of 25 - 400 ng/mL.
Background Cathepsin L (CTSL1) is also known as major excreted protein (MEP), is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains linked by disulfide bonds. CTSL1 is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. MEP has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. CTSL1 is important for the overall degradation of proteins in lysosomes. The specificity of MEP is close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z - Arg - Arg - NHMec, and no peptidyl - dipeptidase activity. Human Cathepsin L activity is greatest under mildly acidic conditions, from pH 4.5 - 6.5. The stability of the enzyme decreases at higher pH values
Molecular Weight 36.7 kDa
NCBI Accession NP_001903
UniProt P07711
Research Area Signaling, Extracellular Matrix, Cancer, Proteases
Pathways Activation of Innate immune Response, Toll-Like Receptors Cascades
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Sodium Acetate, 100 mM NaCl, pH 5.0
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Cathepsin L1 (CTSL1) (AA 18-333) (Active) protein (His tag) (ABIN2180948) Human Cathepsin L, His Tag on SDS-PAGE under reducing (R) condition. The gel was stai...
Background publications Cailhier, Sirois, Laplante, Lepage, Raymond, Brassard, Prat, Iozzo, Pshezhetsky, Hébert: "Caspase-3 activation triggers extracellular cathepsin L release and endorepellin proteolysis." in: The Journal of biological chemistry, Vol. 283, Issue 40, pp. 27220-9, 2008 (PubMed).

Goretzki, Schmitt, Mann, Calvete, Chucholowski, Kramer, Günzler, Jänicke, Graeff: "Effective activation of the proenzyme form of the urokinase-type plasminogen activator (pro-uPA) by the cysteine protease cathepsin L." in: FEBS letters, Vol. 297, Issue 1-2, pp. 112-8, 1992 (PubMed).