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  • 产品名称：MAD2MitoticArrestDeficient-Like1(Yeast)(MAD2L1)(AA1-205)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
MAD2MitoticArrestDeficient-Like1(Yeast)(MAD2L1)(AA1-205)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 24.3 kDa. The protein migrates as 24 kDa under reducing (R) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Mitotic arrest deficient 2-like protein 1 (MAD2L1) is also known as Mitotic spindle assembly checkpoint protein MAD2A (HsMAD2), MAD2. MAD2L1 / MAD2 belongs to the MAD2 family. MAD2L1 / MAD2 contains one HORMA domain. MAD2L1 is the component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 / MAD2 is required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.
Molecular Weight 24.3 kDa
NCBI Accession NP_002349
UniProt Q13257
Research Area Cell Cycle
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (AA 1-205) (Active) protein (His tag) (ABIN2181494) Human MAD2L1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained o...
Background publications Luo, Tang, Xia, Wassmann, Matsumoto, Rizo, Yu: "The Mad2 spindle checkpoint protein has two distinct natively folded states." in: Nature structural & molecular biology, Vol. 11, Issue 4, pp. 338-45, 2004 (PubMed).

Luo, Fang, Coldiron, Lin, Yu, Kirschner, Wagner: "Structure of the Mad2 spindle assembly checkpoint protein and its interaction with Cdc20." in: Nature structural biology, Vol. 7, Issue 3, pp. 224-9, 2000 (PubMed).