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  • 产品名称：CathepsinB(CTSB)(AA18-339)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CathepsinB(CTSB)(AA18-339)(Active)protein(Histag)
详情介绍：
Characteristics rh CTSB is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 36.7 kDa (pro-form) and 29 kDa (mature-form). The predicted N-terminus is Arg18 (pro-form) or Phe74 (mature-form). Due to glycosylation, The reducing (R) protein migrates as 43 kDa (pro-form) band in SDS-PAGE and there may be a 34 kDa (mature-form) band.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC. Measured in 100μL reaction mixture containing 25 mM MES, pH 5.0, 0.01 μg rhCathepsin B, 10 μM reaction substrate. The specific activity is >2800 pmol/min/μg.

SDS-PAGE: and there may be a 34 kDa (mature-form) band.
Background Cathepsin B (CTSB) is also known as APP secretase (APPS) and CPSB, is an enzymatic protein belonging to the peptidase C1 family. Cathepsin B / CTSB is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme. The catalytic activity of Cathepsin B / APPS contains: Hydrolysis of proteins with broad specificity for peptide bonds, Preferentially cleaves -Arg-Arg-|-Xaa bonds in small molecule substrates (thus differing from cathepsin L), In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides. As a thiol protease, cathepsin B / CPSB is believed to participate in intracellular degradation and turnover of proteins and has also been implicated in tumor invasion and metastasis. Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors.
Molecular Weight 36.7 kDa (pro-form) and 29 kDa (mature-form)
NCBI Accession NP_001899
UniProt P07858
Research Area Signaling, Extracellular Matrix, Proteases
Pathways Activation of Innate immune Response, Toll-Like Receptors Cascades
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 150 mM NaCl, pH 8.0
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Cathepsin B (CTSB) (AA 18-339) (Active) protein (His tag) (ABIN2180942) Human Cathepsin B, His Tag on SDS-PAGE under reducing (R) condition. The gel was stai...
Background publications Royer-Zemmour, Ponsole-Lenfant, Gara, Roll, Lévêque, Massacrier, Ferracci, Cillario, Robaglia-Schlupp, Vincentelli, Cau, Szepetowski: "Epileptic and developmental disorders of the speech cortex: ligand/receptor interaction of wild-type and mutant SRPX2 with the plasminogen activator receptor uPAR." in: Human molecular genetics, Vol. 17, Issue 23, pp. 3617-30, 2008 (PubMed).

Chi, Valencia, Hu, Watabe, Yamaguchi, Mangini, Huang, Canfield, Cheng, Yang, Abe, Yamagishi, Shabanowitz, Hearing, Wu, Appella, Hunt: "Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes." in: Journal of proteome research, Vol. 5, Issue 11, pp. 3135-44, 2006 (PubMed).

Basrur, Yang, Kushimoto, Higashimoto, Yasumoto, Valencia, Muller, Vieira, Watabe, Shabanowitz, Hearing, Hunt, Appella: "Proteomic analysis of early melanosomes: identification of novel melanosomal proteins." in: Journal of proteome research, Vol. 2, Issue 1, pp. 69-79, 2003 (PubMed).