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  • 产品名称：CD34Molecule(CD34)(AA32-290)(Active)protein(FcTag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CD34Molecule(CD34)(AA32-290)(Active)protein(FcTag)
详情介绍：
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 53.6 kDa. The protein migrates as 100 kDa and 120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background CD34 Molecule is a cluster of differentiation molecule present on certain cells within the human body. It is a cell surface glycoprotein and functions as a cell-cell adhesion factor. It may also mediate the attachment of stem cells to bone marrow extracellular matrix or directly to stromal cells. As a ~ 110 kDa monomeric cell surface antigen, CD34 is highly glycosylated with nine potential N-linked and numerous potential O-linked glycosylation sites in its extracellular domain. The CD34 protein is a member of a family of single-pass transmembrane sialomucin proteins that show expression on early hematopoietic and vascular-associated tissue. CD34 is also an important adhesion molecule and is required for T cells to enter lymph nodes. It is expressed on lymph node endothelia whereas the L-selectin to which it binds is on the T cell. It was indicated that CD34 is a phosphorylation target for activated PKC, and couples to the hematopoietic adapter protein CrkL, which were involved in CD34 signaling pathways. CD34 is abberantly expressed in many kinds of tumors and is implicated in leukemogenesis.
Molecular Weight 53.6 kDa
NCBI Accession NP_001020280
UniProt P28906
Research Area Hematopoietic Progenitors, Hematopoietic Stem Cells, Cardiogenesis, Lineage Markers, CD Antigens, Surface Receptors of Immune Cells
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for CD34 Molecule (CD34) (AA 32-290) (Active) protein (Fc Tag) (ABIN2180760) CD34 Molecule (CD34) (AA 32-290) (Active) protein (Fc Tag)
Background publications Melrose, Tsurushita, Liu, Berg: "IFN-gamma inhibits activation-induced expression of E- and P-selectin on endothelial cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 5, pp. 2457-64, 1998 (PubMed).

Simmons, Satterthwaite, Tenen, Seed: "Molecular cloning of a cDNA encoding CD34, a sialomucin of human hematopoietic stem cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 1, pp. 267-71, 1992 (PubMed).