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  • 产品名称：HLA-DR-gamma(CD74)(AA73-232)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
HLA-DR-gamma(CD74)(AA73-232)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 19.3 kDa. The protein migrates as 23-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD74 at 5 μg/mL ( 100 μL/well ) can bind biotinylated human CTSL1 with a linear range of 3.2 - 500 ng/mL.
Background Cluster of Differentiation 74 (CD74) is also known as HLA class II histocompatibility antigen gamma chain (HLADG), DHLAG, Ia-GAMMA, invariant chain (Abbreviated Ii). CD74 / HLADG is a polypeptide involved in the formation and transport of MHC class II protein. The cell surface form of the invariant chain is known as CD74. CD74 is upregulated in several cancers and is also expressed by non-immune cells during inflammation. CD74 plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha / beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal / lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. CD74 / DHLAG also serves as cell surface receptor for the cytokine MIF.
Molecular Weight 19.3 kDa
NCBI Accession NP_004346
UniProt P04233
Research Area Immunology, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Major Histocompatibility Complex (MHC)
Pathways Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for HLA-DR-gamma (CD74) (AA 73-232) (Active) protein (His tag) (ABIN2180835) Human CD74, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ove...
Background publications Leng, Metz, Fang, Xu, Donnelly, Baugh, Delohery, Chen, Mitchell, Bucala: "MIF signal transduction initiated by binding to CD74." in: The Journal of experimental medicine, Vol. 197, Issue 11, pp. 1467-76, 2003 (PubMed).

Cresswell: "Assembly, transport, and function of MHC class II molecules." in: Annual review of immunology, Vol. 12, pp. 259-93, 1994 (PubMed).

Scanlon: "The evolution of surgical oncology. Presidential address: James Ewing society, New Orleans, 1975." in: Cancer, Vol. 37, Issue 1, pp. 58-61, 1976 (PubMed).