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  • 产品名称：CD40Molecule,TNFReceptorSuperfamilyMember5(CD40)(AA21-193)(Active)protein(FcTag

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  • 产品厂商：ACROBiosystems
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简单介绍：
CD40Molecule,TNFReceptorSuperfamilyMember5(CD40)(AA21-193)(Active)protein(FcTag)
详情介绍：
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 45.3 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized rhCD40-Fc at 2 μg/mL ( 100 μL/well ) can bind biotinylated human CD40L with a linear range of 7.8 - 125 ng/mL.

SDS-PAGE: resulting from glycosylation.
Background CD40 is also known as TNFRSF5, Bp50, CDW40, MGC9013, TNFRSF5 and p50, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins, and plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. CD40 contains 4 cysteine-rich repeats in the extracellular domain, and is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. The extracellular domain has the cysteinerich repeat regions, which are characteristic for many of the receptors of the TNF superfamily. Interaction of CD40 with its ligand, CD40L, leads to aggregation of CD40 Molecules,which in turn interact with cytoplasmic components to initiate signaling pathways. Early studies on the CD40-CD40L system revealed its role in humoral immunity. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3), an autosomal recessive disorder characterized by an inability of B cells to undergo isotype switching, as well as an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
Molecular Weight 45.3 kDa
NCBI Accession NP_001241
UniProt P25942
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Inflammation, Cancer
Pathways NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (AA 21-193) (Active) protein (Fc Tag) (ABIN2859199) Human CD40, Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel ...
Binding Studies (Bind) image for CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (AA 21-193) (Active) protein (Fc Tag) (ABIN2859199) Immobilized Human CD40, Fc Tag (HPLC-verified) (Cat# CD0-H5253) at 2 μg/mL (100 μl/we...
Background publications Bhushan, Covey: "CREB/ATF proteins enhance the basal and CD154- and IL-4-induced transcriptional activity of the human Igamma1 proximal promoter." in: European journal of immunology, Vol. 31, Issue 2, pp. 653-64, 2001 (PubMed).

Ferrari, Giliani, Insalaco, Al-Ghonaium, Soresina, Loubser, Avanzini, Marconi, Badolato, Ugazio, Levy, Catalan, Durandy, Tbakhi, Notarangelo, Plebani: "Mutations of CD40 gene cause an autosomal recessive form of immunodeficiency with hyper IgM." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 22, pp. 12614-9, 2001 (PubMed).

Bhushan, Covey: "CD40:CD40L interactions in X-linked and non-X-linked hyper-IgM syndromes." in: Immunologic research, Vol. 24, Issue 3, pp. 311-24, 2001 (PubMed).

Ni, Welsh, Leo, Chiou, Wu, Reed, Ely: "Molecular basis for CD40 signaling mediated by TRAF3." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 19, pp. 10395-9, 2000 (PubMed).

Banchereau, Bazan, Blanchard, Brière, Galizzi, van Kooten, Liu, Rousset, Saeland: "The CD40 antigen and its ligand." in: Annual review of immunology, Vol. 12, pp. 881-922, 1994 (PubMed).