产品详情
查看大图

  • 产品名称：CD274Molecule(CD274)(AA19-238)(Active)protein(Histag)

  • 产品型号：
  • 产品厂商：ACROBiosystems
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
CD274Molecule(CD274)(AA19-238)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26 kDa. The protein migrates as 35-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >98 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Grade HPLC verified
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA.Immobilized Human PDL1 /CD274 Protein (Cat# PD1-H5229) at 5μg/mL (100 μL/well),can bind Biotinylated Human PDCD1 Protein, C-Fc, His & AVI Tagged (Cat# PD1-H82F2).The EC50 is typically 0.16-2.4 μg/mL. Measured by its binding ability in a functional ELISA. Immobilized rh PD-L1 / B7-H1 (Catalog # PD1-H5229) at 2 μg/mL ( 100 μL/well ) can bind rhPD1/PDCD1 Fc Chimera (Catalog # PD1-H5257) with a linear range of 0.05-1 μg/mL. Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is typically 0.6-5 μg/mL.

SDS-PAGE: due to glycosylation.
Background Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.
Molecular Weight 26 kDa
NCBI Accession NP_054862
UniProt Q9NZQ7
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for CD274 Molecule (CD274) (AA 19-238) (Active) protein (His tag) (ABIN2181639) Human PD-L1, His Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The ge...
Binding Studies (Bind) image for CD274 Molecule (CD274) (AA 19-238) (Active) protein (His tag) (ABIN2181639) Immobilized Human PD-L1, His Tag (HPLC-verified) with a linear range of 0.05-1 μg/mL.
Background publications Freeman, Long, Iwai, Bourque, Chernova, Nishimura, Fitz, Malenkovich, Okazaki, Byrne, Horton, Fouser, Carter, Ling, Bowman, Carreno, Collins, Wood, Honjo: "Engagement of the PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation." in: The Journal of experimental medicine, Vol. 192, Issue 7, pp. 1027-34, 2000 (PubMed).

Dong, Zhu, Tamada, Chen: "B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion." in: Nature medicine, Vol. 5, Issue 12, pp. 1365-9, 1999 (PubMed).