产品详情
查看大图

  • 产品名称：CathepsinD(CTSD)(AA19-412)(Active)protein(Histag)

  • 产品型号：
  • 产品厂商：ACROBiosystems
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
CathepsinD(CTSD)(AA19-412)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 43.6 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is >450 pmol/min/μg.
Background Cathepsin D is also known as CTSD, CPSD, which belongs to the peptidase A1 family. Cathepsin D can be cleaved into the following 2 chains: cathepsin D light chain and cathepsin D heavy chain, which is expressed in the aorta extrcellular space (at protein level). The catalytic activity of Cathepsin D is specificity similar to, but narrower than, that of pepsin A. Cathepsin D does not cleave the 4-Gln-|-His-5 bond in B chain of insulin. Cathepsin D involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
Molecular Weight 43.6 kDa
NCBI Accession NP_001900
UniProt P07339
Research Area Proteolysis / Ubiquitin, Extracellular Matrix, Proteases, Signaling
Pathways Peptide Hormone Metabolism
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM MES, 100 mM NaCl, pH 6.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Cathepsin D (CTSD) (AA 19-412) (Active) protein (His tag) (ABIN2180944) Human Cathepsin D, His Tag on SDS-PAGE under reducing (R) condition. The gel was stai...
Background publications Didangelos, Yin, Mandal, Baumert, Jahangiri, Mayr: "Proteomics characterization of extracellular space components in the human aorta." in: Molecular & cellular proteomics : MCP, Vol. 9, Issue 9, pp. 2048-62, 2010 (PubMed).

Zhang, Li, Martin, Aebersold: "Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry." in: Nature biotechnology, Vol. 21, Issue 6, pp. 660-6, 2003 (PubMed).

Papassotiropoulos, Bagli, Kurz, Kornhuber, Förstl, Maier, Pauls, Lautenschlager, Heun: "A genetic variation of cathepsin D is a major risk factor for Alzheimer's disease." in: Annals of neurology, Vol. 47, Issue 3, pp. 399-403, 2000 (PubMed).